論文 - 詳細
| RRC ID | 85162 |
|---|---|
| 著者 | Leroy E, Challal D, Pelletier S, Goncalves C, Menant A, Marchand V, Jaszczyszyn Y, van Dijk E, Naquin D, Andreani J, Motorin Y, Palancade B, Rougemaille M. |
| タイトル | A bifunctional snoRNA with separable activities in guiding rRNA 2'-O-methylation and scaffolding gametogenesis effectors. |
| ジャーナル | Nat Commun |
| Abstract |
Small nucleolar RNAs are non-coding transcripts that guide chemical modifications of RNA substrates and modulate gene expression at the epigenetic and post-transcriptional levels. However, the extent of their regulatory potential and the underlying molecular mechanisms remain poorly understood. Here, we identify a conserved, previously unannotated intronic C/D-box snoRNA, termed snR107, hosted in the fission yeast long non-coding RNA mamRNA and carrying two independent cellular functions. On the one hand, snR107 guides site-specific 25S rRNA 2'-O-methylation and promotes pre-rRNA processing and 60S subunit biogenesis. On the other hand, snR107 associates with the gametogenic RNA-binding proteins Mmi1 and Mei2, mediating their reciprocal inhibition and restricting meiotic gene expression during sexual differentiation. Both functions require distinct cis-motifs within snR107, including a conserved 2'-O-methylation guiding sequence. Together, our results position snR107 as a dual regulator of rRNA modification and gametogenesis effectors, expanding our vision on the non-canonical functions exerted by snoRNAs in cell fate decisions. |
| 巻・号 | 16(1) |
| ページ | 3250 |
| 公開日 | 2025-4-5 |
| DOI | 10.1038/s41467-025-58664-y |
| PII | 10.1038/s41467-025-58664-y |
| PMID | 40185772 |
| PMC | PMC11971394 |
| MeSH | Gametogenesis* / genetics Gene Expression Regulation, Fungal Methylation RNA Precursors / genetics RNA Precursors / metabolism RNA Processing, Post-Transcriptional RNA, Fungal / genetics RNA, Fungal / metabolism RNA, Long Noncoding / genetics RNA, Long Noncoding / metabolism RNA, Ribosomal* / genetics RNA, Ribosomal* / metabolism RNA, Small Nucleolar* / genetics RNA, Small Nucleolar* / metabolism RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism Schizosaccharomyces* / genetics Schizosaccharomyces* / metabolism Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism mRNA Cleavage and Polyadenylation Factors |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 18 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FY39932 |