| RRC ID |
85162
|
| 著者 |
Leroy E, Challal D, Pelletier S, Goncalves C, Menant A, Marchand V, Jaszczyszyn Y, van Dijk E, Naquin D, Andreani J, Motorin Y, Palancade B, Rougemaille M.
|
| タイトル |
A bifunctional snoRNA with separable activities in guiding rRNA 2'-O-methylation and scaffolding gametogenesis effectors.
|
| ジャーナル |
Nat Commun
|
| Abstract |
Small nucleolar RNAs are non-coding transcripts that guide chemical modifications of RNA substrates and modulate gene expression at the epigenetic and post-transcriptional levels. However, the extent of their regulatory potential and the underlying molecular mechanisms remain poorly understood. Here, we identify a conserved, previously unannotated intronic C/D-box snoRNA, termed snR107, hosted in the fission yeast long non-coding RNA mamRNA and carrying two independent cellular functions. On the one hand, snR107 guides site-specific 25S rRNA 2'-O-methylation and promotes pre-rRNA processing and 60S subunit biogenesis. On the other hand, snR107 associates with the gametogenic RNA-binding proteins Mmi1 and Mei2, mediating their reciprocal inhibition and restricting meiotic gene expression during sexual differentiation. Both functions require distinct cis-motifs within snR107, including a conserved 2'-O-methylation guiding sequence. Together, our results position snR107 as a dual regulator of rRNA modification and gametogenesis effectors, expanding our vision on the non-canonical functions exerted by snoRNAs in cell fate decisions.
|
| 巻・号 |
16(1)
|
| ページ |
3250
|
| 公開日 |
2025-4-5
|
| DOI |
10.1038/s41467-025-58664-y
|
| PII |
10.1038/s41467-025-58664-y
|
| PMID |
40185772
|
| PMC |
PMC11971394
|
| MeSH |
Gametogenesis* / genetics
Gene Expression Regulation, Fungal
Methylation
RNA Precursors / genetics
RNA Precursors / metabolism
RNA Processing, Post-Transcriptional
RNA, Fungal / genetics
RNA, Fungal / metabolism
RNA, Long Noncoding / genetics
RNA, Long Noncoding / metabolism
RNA, Ribosomal* / genetics
RNA, Ribosomal* / metabolism
RNA, Small Nucleolar* / genetics
RNA, Small Nucleolar* / metabolism
RNA-Binding Proteins / genetics
RNA-Binding Proteins / metabolism
Schizosaccharomyces* / genetics
Schizosaccharomyces* / metabolism
Schizosaccharomyces pombe Proteins / genetics
Schizosaccharomyces pombe Proteins / metabolism
mRNA Cleavage and Polyadenylation Factors
|
| IF |
12.121
|
| リソース情報 |
| 酵母 |
FY39932 |
