論文 - 詳細
| RRC ID | 85220 |
|---|---|
| 著者 | Deng X, Higaki T, Lin HH, Lee YJ, Liu B. |
| タイトル | The unconventional TPX2 family protein TPXL3 regulates α Aurora kinase function in spindle morphogenesis in Arabidopsis. |
| ジャーナル | Plant Cell |
| Abstract |
Spindle assembly in vertebrates requires the Aurora kinase, which is targeted to microtubules and activated by TPX2 (Targeting Protein of XKLP2). In Arabidopsis (Arabidopsis thaliana), TPX2-LIKE 3 (TPXL3), but not the highly conserved TPX2, is essential. To test the hypothesis that TPXL3 regulates the function of α Aurora kinase in spindle assembly, we generated transgenic Arabidopsis lines expressing an artificial microRNA targeting TPXL3 mRNA (amiR-TPXL3). The resulting mutants exhibited growth retardation, which was linked to compromised TPXL3 expression. In the mutant cells, α Aurora was delocalized from spindle microtubules to the cytoplasm, and spindles were assembled without recognizable poles. A functional TPXL3-GFP fusion protein first prominently appeared on the prophase nuclear envelope. Then, TPXL3-GFP localized to spindle microtubules (primarily toward the spindle poles, like γ-tubulin), and finally to the re-forming nuclear envelope during telophase and cytokinesis. However, TPXL3 was absent from phragmoplast microtubules. In addition, we found that the TPXL3 N-terminal Aurora-binding motif, microtubule-binding domain, and importin-binding motif, but not the C-terminal segment, were required for its mitotic function. Expression of truncated TPXL3 variants enhanced the defects in spindle assembly and seedling growth of amiR-TPXL3 plants. Taken together, our findings uncovered the essential function of TPXL3, but not TPX2, in targeting and activating α Aurora kinase for spindle apparatus assembly in Arabidopsis. |
| 巻・号 | 37(4) |
| 公開日 | 2025-4-2 |
| DOI | 10.1093/plcell/koaf065 |
| PII | 8096453 |
| PMID | 40139933 |
| PMC | PMC12012799 |
| MeSH | Arabidopsis* / cytology Arabidopsis* / genetics Arabidopsis* / growth & development Arabidopsis* / metabolism Arabidopsis Proteins* / genetics Arabidopsis Proteins* / metabolism Aurora Kinases* / genetics Aurora Kinases* / metabolism Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Gene Expression Regulation, Plant Microtubule-Associated Proteins* / genetics Microtubule-Associated Proteins* / metabolism Microtubules / metabolism Mutation Nuclear Envelope / metabolism Plants, Genetically Modified Spindle Apparatus* / metabolism |
| IF | 9.618 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 19 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda17343 |