| RRC ID |
85431
|
| 著者 |
Dong A, Yoshizumi M, Kokubo H.
|
| タイトル |
Odz4 upregulates SAN-specific genes to promote differentiation into cardiac pacemaker-like cells.
|
| ジャーナル |
FEBS Lett
|
| Abstract |
Cardiac arrhythmias stemming from abnormal sinoatrial node (SAN) function can lead to sudden death. Developing a biological pacemaker device for treating sick sinus syndrome (SSS) could offer a potential cure. Understanding SAN differentiation is crucial, yet its regulatory mechanism remains unclear. We reanalyzed published RNA-seq data and identified Odz4 as a SAN-specific candidate. In situ hybridization revealed Odz4 expression in the cardiac crescent and throughout the cardiac conduction system (CCS). To assess the role of Odz4 in CCS differentiation, we utilized a Tet-Off inducible system for its intracellular domain (ICD). Embryonic bodies (EBs) exogenously expressing Odz4-ICD exhibited an increased propensity to develop into pacemaker-like cells with enhanced automaticity and upregulated expression of SAN-specific genes. CellChat and GO analyses unveiled SAN-specific enrichment of ligand-receptor sets, especially Ptn-Ncl, and extracellular matrix components in the group exogenously expressing Odz4-ICD. Our findings underscore the significance of Odz4 in SAN development and offer fresh insights into biological pacemaker establishment.
|
| 巻・号 |
599(3)
|
| ページ |
299-315
|
| 公開日 |
2025-2-1
|
| DOI |
10.1002/1873-3468.15036
|
| PMID |
39462648
|
| PMC |
PMC11808419
|
| MeSH |
Animals
Cell Differentiation* / genetics
Humans
Mice
Myocytes, Cardiac* / cytology
Myocytes, Cardiac* / metabolism
Sick Sinus Syndrome / genetics
Sick Sinus Syndrome / metabolism
Sinoatrial Node* / cytology
Sinoatrial Node* / metabolism
Up-Regulation
|
| IF |
3.057
|
| リソース情報 |
| ヒト・動物細胞 |
COS-7(RCB0539)
EBRTcH3(AES0054) |
