Reference - Detail
| RRC ID | 85454 |
|---|---|
| Author | Ichikawa T, Hiono T, Okamatsu M, Maruyama J, Kobayashi D, Matsuno K, Kida H, Sakoda Y. |
| Title | Hemagglutinin and neuraminidase of a non-pathogenic H7N7 avian influenza virus coevolved during the acquisition of intranasal pathogenicity in chickens. |
| Journal | Arch Virol |
| Abstract |
Polybasic amino acid residues at the hemagglutinin (HA) cleavage site are insufficient to induce the highly pathogenic phenotype of avian influenza viruses in chickens. In our previous study, an H7N7 avian influenza virus named "Vac2sub-P0", which is nonpathogenic despite carrying polybasic amino acids at the HA cleavage site, was passaged in chick air sacs, and a virus with high intravenous pathogenicity, Vac2sub-P3, was obtained. Intranasal infection with Vac2sub-P3 resulted in limited lethality in chickens; therefore, in this study, this virus was further passaged in chicken lungs, and the resultant virus, Vac2sub-P3L4, acquired high intranasal pathogenicity. Experimental infection of chickens with recombinant viruses demonstrated that mutations in HA and neuraminidase (NA) found in consecutive passages were responsible for the increased pathogenicity. The HA and NA functions of Vac2sub-P3L4 were compared with those of the parental virus in vitro; the virus growth at 40 °C was faster, the binding affinity to a sialic acid receptor was lower, and the rate of release by NA from the cell surface was lower, suggesting that these changes enabled the virus to replicate efficiently in chickens with high intranasal pathogenicity. This study demonstrates that viruses that are highly pathogenic when administered intranasally require additional adaptations for increased pathogenicity to be highly lethal to intranasally infected chickens. |
| Volume | 169(10) |
| Pages | 207 |
| Published | 2024-9-22 |
| DOI | 10.1007/s00705-024-06118-z |
| PII | 10.1007/s00705-024-06118-z |
| PMID | 39307848 |
| MeSH | Animals Chickens* / virology Evolution, Molecular Hemagglutinin Glycoproteins, Influenza Virus* / genetics Hemagglutinin Glycoproteins, Influenza Virus* / metabolism Influenza A Virus, H7N7 Subtype* / genetics Influenza A Virus, H7N7 Subtype* / pathogenicity Influenza in Birds* / virology Mutation Neuraminidase* / genetics Neuraminidase* / metabolism Poultry Diseases / virology Viral Proteins / genetics Viral Proteins / metabolism Virulence |
| IF | 2.243 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 10 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human pathogenic viruses | Vac2sub-P0 |