RRC ID 859
Author Shimuta TR, Nakano K, Yamaguchi Y, Ozaki S, Fujimitsu K, Matsunaga C, Noguchi K, Emoto A, Katayama T.
Title Novel heat shock protein HspQ stimulates the degradation of mutant DnaA protein in Escherichia coli.
Journal Genes Cells
Abstract Escherichia coli DnaA protein initiates chromosomal replication and is an important regulatory target during the replication cycle. In this study, a suppressor mutation isolated by transposon mutagenesis was found to allow growth of the temperature-sensitive dnaA508 and dnaA167 mutants at 40 degrees C. The suppressor consists of a transposon insertion in a previously annotated ORF, here termed hspQ, a novel heat shock gene whose promoter is recognized by the major heat shock sigma factor sigma32. Expression of hspQ on a pBR322 derivative inhibits growth of the dnaA508 and dnaA167 mutants at 30 degrees C, whereas growth of dnaA46 and other dnaA mutants is insensitive to changes in the level of hspQ. Cellular DnaA508 protein is degraded rapidly at elevated temperature, but hspQ disruption impedes this process. In contrast, DnaA46 protein is rapidly degraded in an hspQ-independent manner. Gel-filtration and chemical cross-linking experiments suggest that HspQ forms a stable homodimer in solution and can form homomultimers consisting of about four monomers. Heat-shock induced proteases such as Clp contain homomultimers of subunit proteins. We propose that HspQ is a new factor involved in the quality control of proteins and that it functions by excluding denatured proteins.
Volume 9(12)
Pages 1151-66
Published 2004-12-1
DOI 10.1111/j.1365-2443.2004.00800.x
PMID 15569148
MeSH Alleles Amino Acid Sequence Bacterial Proteins / genetics Bacterial Proteins / metabolism* Base Sequence DNA Replication DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Escherichia coli / genetics* Escherichia coli Proteins / genetics* Escherichia coli Proteins / isolation & purification Escherichia coli Proteins / physiology* Heat-Shock Proteins / genetics* Heat-Shock Proteins / isolation & purification Heat-Shock Proteins / metabolism Heat-Shock Proteins / physiology* Molecular Sequence Data Mutation Protein Denaturation Up-Regulation
IF 1.655
Times Cited 12
Prokaryotes E. coli ME9018