| RRC ID |
859
|
| 著者 |
Shimuta TR, Nakano K, Yamaguchi Y, Ozaki S, Fujimitsu K, Matsunaga C, Noguchi K, Emoto A, Katayama T.
|
| タイトル |
Novel heat shock protein HspQ stimulates the degradation of mutant DnaA protein in Escherichia coli.
|
| ジャーナル |
Genes Cells
|
| Abstract |
Escherichia coli DnaA protein initiates chromosomal replication and is an important regulatory target during the replication cycle. In this study, a suppressor mutation isolated by transposon mutagenesis was found to allow growth of the temperature-sensitive dnaA508 and dnaA167 mutants at 40 degrees C. The suppressor consists of a transposon insertion in a previously annotated ORF, here termed hspQ, a novel heat shock gene whose promoter is recognized by the major heat shock sigma factor sigma32. Expression of hspQ on a pBR322 derivative inhibits growth of the dnaA508 and dnaA167 mutants at 30 degrees C, whereas growth of dnaA46 and other dnaA mutants is insensitive to changes in the level of hspQ. Cellular DnaA508 protein is degraded rapidly at elevated temperature, but hspQ disruption impedes this process. In contrast, DnaA46 protein is rapidly degraded in an hspQ-independent manner. Gel-filtration and chemical cross-linking experiments suggest that HspQ forms a stable homodimer in solution and can form homomultimers consisting of about four monomers. Heat-shock induced proteases such as Clp contain homomultimers of subunit proteins. We propose that HspQ is a new factor involved in the quality control of proteins and that it functions by excluding denatured proteins.
|
| 巻・号 |
9(12)
|
| ページ |
1151-66
|
| 公開日 |
2004-12-1
|
| DOI |
10.1111/j.1365-2443.2004.00800.x
|
| PII |
GTC800
|
| PMID |
15569148
|
| MeSH |
Alleles
Amino Acid Sequence
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Base Sequence
DNA Replication
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism*
Escherichia coli / genetics*
Escherichia coli Proteins / genetics*
Escherichia coli Proteins / isolation & purification
Escherichia coli Proteins / physiology*
Heat-Shock Proteins / genetics*
Heat-Shock Proteins / isolation & purification
Heat-Shock Proteins / metabolism
Heat-Shock Proteins / physiology*
Molecular Sequence Data
Mutation
Protein Denaturation
Up-Regulation
|
| IF |
1.655
|
| 引用数 |
12
|
|
WOS 分野
|
GENETICS & HEREDITY
CELL BIOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
ME9018 |
