| RRC ID |
86109
|
| 著者 |
Kurinami S, Fukui K, Murakawa T, Baba S, Kumasaka T, Okanishi H, Kanai Y, Yano T, Masui R.
|
| タイトル |
The crystal structure of the small zinc-finger protein ZifS from Thermus thermophilus HB8.
|
| ジャーナル |
J Biochem
|
| Abstract |
Zinc finger domains are important interaction modules for binding to nucleic acids, proteins, lipids and small molecules. Many small-sized zinc finger proteins are encoded in bacterial genomes, but most of them have not been functionally annotated. We focused on TTHA0897, ZifS, as a small zinc finger protein from the extremely thermophilic eubacterium Thermus thermophilus HB8. In vivo experiments suggested that the cellular function of ZifS is related to the growth transition of T. thermophilus from the lag to the exponential phase under nutritionally limited conditions. In vitro biochemical experiments, including electrophoretic mobility shift assay and pull-down assay, yielded no clues about molecular functions of ZifS. X-ray crystallographic analysis revealed that the dimeric ZifS globally forms a cylinder-like structure, although ZifS dimer has no overall structural similarity to other known zinc finger proteins. The zinc ion-binding manner of ZifS fitted the characteristics of the zinc ribbon fold, which are mostly found in domains from proteins involved in the transcriptional and translational machinery. The crystal structure of ZifS is the first experimental insight into the molecular structure of this protein family, revealing several conserved features that may be functionally relevant.
|
| 巻・号 |
178(2)
|
| ページ |
121-133
|
| 公開日 |
2025-7-31
|
| DOI |
10.1093/jb/mvaf028
|
| PII |
8153023
|
| PMID |
40441711
|
| MeSH |
Amino Acid Sequence
Bacterial Proteins* / chemistry
Bacterial Proteins* / genetics
Bacterial Proteins* / metabolism
Crystallography, X-Ray
Models, Molecular
Protein Conformation
Thermus thermophilus* / chemistry
Thermus thermophilus* / metabolism
Zinc / metabolism
Zinc Fingers*
|
| IF |
2.476
|
| リソース情報 |
| 遺伝子材料 |
Thermus thermophilus HB8 gene disruption plasmid
TDs05F05 (THR022125)
TDs05E05 (THR022101)
Thermus thermophilus expression plasmid
TEx01B02 (THR000426)
TEx14A09 (THR005609) |
