RRC ID 862
Author Hasegawa Y, Wakabayashi M, Nakamura S, Kodaira K, Shinohara H, Yasukawa H.
Title A homolog of Escherichia coli RecA in mitochondria of the cellular slime mold Dictyostelium discoideum.
Journal DNA Repair (Amst)
Abstract The cellular slime mold Dictyostelium discoideum expresses a gene encoding a 452-amino-acid polypeptide that is 47% identical to Escherichia coli RecA. A recA-deficient E. coli, JE6651, was transformed by pYSN1, which was designed to express the truncated form of the D. discoideum gene, and used in suppression assays. The viability of the transformant, JE6651(pYSN1), increased following UV irradiation or mitomycin C treatment. Phage lambda (red(-) gam(-)), which required RecA activity for DNA packaging, formed plaques on a lawn of JE6651(pYSN1). These results indicate that the gene product has a DNA recombination activity. Fluorescence of D. discoideum protein fused with GFP was detected in mitochondria. The gene disruption mutant was hypersensitive to UV-light (254nm), mitomycin C and H(2)O(2), indicating that D. discoideum recA is important for survival following exposure to DNA damaging agents.
Volume 3(5)
Pages 515-25
Published 2004-5-4
DOI 10.1016/j.dnarep.2004.01.014
PII S1568786404000266
PMID 15084313
MeSH Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA Damage / drug effects DNA Damage / radiation effects Dictyostelium / cytology Dictyostelium / drug effects Dictyostelium / enzymology* Dictyostelium / genetics Escherichia coli / enzymology* Escherichia coli / genetics Hydrogen Peroxide / pharmacology Mitochondria / metabolism* Molecular Sequence Data Mutation / genetics Protein Transport Rec A Recombinases / chemistry Rec A Recombinases / genetics* Rec A Recombinases / metabolism* Ultraviolet Rays
IF 3.339
Times Cited 5
Prokaryotes E. coli JE6650 JE6651
Cellular slime molds G01123 S00328