論文 - 詳細
| RRC ID | 86308 |
|---|---|
| 著者 | Markowska K, Litwin I, Misiorna D, Kończak J, Bogdańska A, Tomaszewska P, Tracz M, Haenen M, Kramarz K. |
| タイトル | PolySUMOylation of PCNA and Rad52 restricts centromeric recombination in fission yeast. |
| ジャーナル | Nat Commun |
| Abstract |
SUMOylation, a conserved post-translational modification in eukaryotes, regulates protein function, localization, and stability. However, the role of SUMO chains in genome maintenance is still emerging. Using Schizosaccharomyces pombe, we show that loss of SUMO chains results in spontaneous replication stress, DNA damage, and elevated centromeric recombination. To investigate SUMO-dependent interactome at the sites of Rad52 repair, we used a split-SUMO-ID proteomics approach. It allows the analysis of local SUMOylation content at the Rad52 repair sites, and enabled the identification of the essential replication factor PCNA. We found that SUMO chain-modified PCNA antagonizes Rad8-mediated PCNA polyubiquitination, modulating the choice of post-replication repair pathways at stalled forks within centromeres. In the absence of polySUMOylation, excessive PCNA polyubiquitination drives elevated recombination at centromeres. Artificial tethering of a SUMO chain to Rad52 suppresses this defect. Our findings uncover an essential role for SUMO chains in centromere maintenance by modulating DNA repair pathway choice under endogenous replication stress. |
| 巻・号 | 16(1) |
| ページ | 10837 |
| 公開日 | 2025-12-2 |
| DOI | 10.1038/s41467-025-65862-1 |
| PII | 10.1038/s41467-025-65862-1 |
| PMID | 41330900 |
| PMC | PMC12672585 |
| MeSH | Centromere* / genetics Centromere* / metabolism DNA Damage DNA Repair DNA Replication DNA-Binding Proteins Proliferating Cell Nuclear Antigen* / genetics Proliferating Cell Nuclear Antigen* / metabolism Rad52 DNA Repair and Recombination Protein* / genetics Rad52 DNA Repair and Recombination Protein* / metabolism Recombination, Genetic* Schizosaccharomyces* / genetics Schizosaccharomyces* / metabolism Schizosaccharomyces pombe Proteins* / genetics Schizosaccharomyces pombe Proteins* / metabolism Sumoylation* Ubiquitination |
| IF | 12.121 |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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