論文 - 詳細
| RRC ID | 86322 |
|---|---|
| 著者 | Iwasaki M, Yamamoto M, Tomihari M, Ishikawa M. |
| タイトル | Ropivacaine Administration Suppressed A549 Lung Adenocarcinoma Cell Proliferation and Migration via ACE2 Upregulation and Inhibition of the Wnt1 Pathway. |
| ジャーナル | Int J Mol Sci |
| Abstract |
BACKGROUND:Previous studies have suggested that perioperative anesthesia could have direct impacts on cancer cell biology. The present study investigated the effects of ropivacaine administration on lung adenocarcinoma cells. METHODS:Ropivacaine was administered to A549 cells at concentrations of 0.1, 1, and 6 mM for 2 h. Angiotensin-converting enzyme 2 (ACE2) small interfering RNA (siRNA) transfection was performed 6 h prior to ropivacaine administration. Cell proliferation and migration were assessed with cell counting kit 8 (CCK-8) and a wound healing assay at 0 and 24 h after anesthesia exposure. PCR arrays were performed, followed by PCR validation. RESULTS:Ropivacaine administration inhibited A549 cell proliferation and migration in a concentration-dependent manner, with ACE2 upregulation and HIF1α (hypoxia-inducible factor 1α) downregulation. The anticancer effect of ropivacaine was canceled out via ACE2 siRNA transfection. PCR arrays showed specific gene change patterns in the ropivacaine and respective ACE2-knockdown groups. EGFR (epidermal growth factor receptor), BAX (Bcl-2-associated X protein) and BCL2 (B-cell/CLL lymphoma 2) were suppressed with ropivacaine administration; these effects were reversed via ACE2 siRNA induction. CONCLUSION:Ropivacaine administration inhibited A549 cell biology in conjunction with ACE2 upregulation via the inhibition of the Wnt1 (wingless/Integrated 1) pathway. |
| 巻・号 | 25(17) |
| 公開日 | 2024-8-28 |
| DOI | 10.3390/ijms25179334 |
| PII | ijms25179334 |
| PMID | 39273283 |
| PMC | PMC11395614 |
| MeSH | A549 Cells Adenocarcinoma of Lung* / drug therapy Adenocarcinoma of Lung* / genetics Adenocarcinoma of Lung* / metabolism Adenocarcinoma of Lung* / pathology Angiotensin-Converting Enzyme 2* / genetics Angiotensin-Converting Enzyme 2* / metabolism Cell Movement* / drug effects Cell Proliferation* / drug effects Gene Expression Regulation, Neoplastic* / drug effects Humans Lung Neoplasms* / drug therapy Lung Neoplasms* / genetics Lung Neoplasms* / metabolism Lung Neoplasms* / pathology Ropivacaine* / pharmacology Up-Regulation / drug effects Wnt Signaling Pathway / drug effects Wnt1 Protein / genetics Wnt1 Protein / metabolism |
| IF | 4.556 |
| リソース情報 | |
| ヒト・動物細胞 | A549(RCB0098) |