論文 - 詳細
| RRC ID | 86574 |
|---|---|
| 著者 | Xie K, Yano S, Wang J, Yamakoshi S, Ohta T, Uto T, Sakai M, He X, Yoshizaki K, Kubota T, Ohnishi K, Hara T. |
| タイトル | The Yeast-Fermented Garlic and a Balance of Spermine/Spermidine Activates Autophagy via EGR1 Transcriptional Factor. |
| ジャーナル | Mol Nutr Food Res |
| Abstract |
Spermine (SPM) and spermidine (SPD) are polyamines found in all organisms, and their concentrations can be regulated by ingestion. We demonstrated that yeast-fermented garlic (YF) extract significantly increased autophag flux in OUMS-36T-1 and HeLa cells expressing the fluorescent probe (GFP-LC3-RFP-LC3ΔG). YF-induced increase of autophagy occurred independently of mTORC1 signaling, and RNA-sequencing analysis revealed that EGR1 was the most significantly altered gene in YF-treated OUMS-36T-1 cells. YF-treated EGR1-deficient HAP1 cells displayed reduced autophagic flux (p < 0.05). YF-induced increasing of autophagic flux occurred via a specific SPM/SPD ratio. HAP1 cells treated with equivalent amounts of SPD or SPM as that found in YF did not increase autophagic flux (p > 0.05); however, treatment with SPD and SPM in the same ratio as that found in YF increased autophagic flux (p < 0.05). This specific SPM/SPD ratio reduced MG132-induced proteostress via EGR1-dependent pathways (p < 0.05). Thus, the SPM/SPD balance may regulate autophagy via EGR1-dependent pathways, and controlling this balance may provide a strategy to maintain cellular homeostasis. |
| 巻・号 | 69(5) |
| ページ | e202400606 |
| 公開日 | 2025-3-1 |
| DOI | 10.1002/mnfr.202400606 |
| PMID | 39945057 |
| PMC | PMC11874185 |
| MeSH | Autophagy* / drug effects Cell Line Early Growth Response Protein 1* / genetics Early Growth Response Protein 1* / metabolism Early Growth Response Protein 1* / physiology Fermentation Garlic* / chemistry HeLa Cells Humans Plant Extracts / pharmacology Signal Transduction Spermidine* / metabolism Spermidine* / pharmacology Spermine* / metabolism Spermine* / pharmacology |
| IF | 5.309 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pMRX-IP-GFP-LC3-RFP-LC3 delta G (RDB14600) pMRX-IP-GFP-LC3-RFP (RDB14601) |