| RRC ID |
86612
|
| 著者 |
Ishii S, Arakawa Y, Ishii H, Yokoyama K, Yokoyama H, Saito T, Yano S.
|
| タイトル |
A novel stroma-dependent leukemia cell line from a patient with mixed-phenotype acute leukemia with Ph chromosome and PAX5 mutation.
|
| ジャーナル |
Int J Hematol
|
| Abstract |
Mixed phenotype acute leukemia (MPAL) is a rare and aggressive form of leukemia with a poor prognosis and no established treatment. In this study, we established a novel leukemic cell line, JMPAL-1, from a specimen of a 69-year-old patient with Philadelphia chromosome-positive MPAL. Flow cytometry showed that JMPAL-1 expresses B-cell markers but not myeloperoxidase. A genomic analysis of JMPAL-1 cells revealed the BCR::ABL1 fusion gene, missense mutation in PAX5, homozygous deletion of CDKN2A/CDKN2B, and BRAF amplification. This cell line was stroma-dependent in proliferation and required co-culturing with mouse bone marrow-derived mesenchymal cells (9-15C). Knowing the differences between JMPAL-1 and patient leukemia cells may improve understanding of the in vivo versus in vitro behavior of leukemia, clonal selection, and transformation. The stroma-dependent growth pattern of JMPAL-1 also provides a unique platform to study tumor-stromal interactions and their role in leukemic cell survival and drug resistance. Our study highlights the importance of establishing preclinical models such as JMPAL-1 and performing detailed cytogenetic analysis to develop targeted therapies in line with the pathogenesis of the disease.
|
| 巻・号 |
121(6)
|
| ページ |
782-791
|
| 公開日 |
2025-6-1
|
| DOI |
10.1007/s12185-025-03944-y
|
| PII |
10.1007/s12185-025-03944-y
|
| PMID |
39979768
|
| MeSH |
Aged
Animals
Cell Line, Tumor
Fusion Proteins, bcr-abl / genetics
Humans
Leukemia, Biphenotypic, Acute* / genetics
Leukemia, Biphenotypic, Acute* / metabolism
Leukemia, Biphenotypic, Acute* / pathology
Male
Mice
Mutation*
PAX5 Transcription Factor* / genetics
Philadelphia Chromosome*
Stromal Cells / metabolism
Stromal Cells / pathology
|
| IF |
2.245
|
| リソース情報 |
| ヒト・動物細胞 |
HKBML(RCB0820) |
