論文 - 詳細
| RRC ID | 86755 |
|---|---|
| 著者 | Lancino M, Majello S, Herbert S, De Chaumont F, Tinevez JY, Olivo-Marin JC, Herbomel P, Schmidt A. |
| タイトル | Anisotropic organization of circumferential actomyosin characterizes hematopoietic stem cells emergence in the zebrafish. |
| ジャーナル | Elife |
| Abstract |
Hematopoiesis leads to the formation of blood and immune cells. Hematopoietic stem cells emerge during development, from vascular components, via a process called the endothelial-to-hematopoietic transition (EHT). Here, we reveal essential biomechanical features of the EHT, using the zebrafish embryo imaged at unprecedented spatio-temporal resolution and an algorithm to unwrap the aorta into 2D-cartography. We show that the transition involves anisotropic contraction along the antero-posterior axis, with heterogenous organization of contractile circumferential actomyosin. The biomechanics of the contraction is oscillatory, with unusually long periods in comparison to other apical constriction mechanisms described so far in morphogenesis, and is supported by the anisotropic reinforcement of junctional contacts. Finally, we show that abrogation of blood flow impairs the actin cytoskeleton, the morphodynamics of EHT cells, and the orientation of the emergence. Overall, our results underline the peculiarities of the EHT biomechanics and the influence of the mechanical forces exerted by blood flow. |
| 巻・号 | 7 |
| 公開日 | 2018-8-22 |
| DOI | 10.7554/eLife.37355 |
| PII | 37355 |
| PMID | 30132756 |
| PMC | PMC6105311 |
| MeSH | Actin Cytoskeleton / metabolism Actins / metabolism Actomyosin / metabolism* Amino Acid Sequence Animals Anisotropy Biomechanical Phenomena Endothelial Cells / cytology Endothelial Cells / metabolism Hematopoiesis Hematopoietic Stem Cells / cytology Hematopoietic Stem Cells / metabolism* Hemodynamics Intercellular Junctions / metabolism Models, Biological Mutation / genetics Myosin Light Chains / metabolism Phenotype Phosphorylation Time Factors Zebrafish / metabolism* |
| IF | 7.08 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 30 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | UAS:RFP |