論文 - 詳細
| RRC ID | 86757 |
|---|---|
| 著者 | Lagendijk AK, Gomez GA, Baek S, Hesselson D, Hughes WE, Paterson S, Conway DE, Belting HG, Affolter M, Smith KA, Schwartz MA, Yap AS, Hogan BM. |
| タイトル | Live imaging molecular changes in junctional tension upon VE-cadherin in zebrafish. |
| ジャーナル | Nat Commun |
| Abstract |
Forces play diverse roles in vascular development, homeostasis and disease. VE-cadherin at endothelial cell-cell junctions links the contractile acto-myosin cytoskeletons of adjacent cells, serving as a tension-transducer. To explore tensile changes across VE-cadherin in live zebrafish, we tailored an optical biosensor approach, originally established in vitro. We validate localization and function of a VE-cadherin tension sensor (TS) in vivo. Changes in tension across VE-cadherin observed using ratio-metric or lifetime FRET measurements reflect acto-myosin contractility within endothelial cells. Furthermore, we apply the TS to reveal biologically relevant changes in VE-cadherin tension that occur as the dorsal aorta matures and upon genetic and chemical perturbations during embryonic development. |
| 巻・号 | 8(1) |
| ページ | 1402 |
| 公開日 | 2017-11-10 |
| DOI | 10.1038/s41467-017-01325-6 |
| PII | 10.1038/s41467-017-01325-6 |
| PMID | 29123087 |
| PMC | PMC5680264 |
| MeSH | Actomyosin / physiology Animals Antigens, CD / genetics Antigens, CD / physiology* Aorta / embryology Biomechanical Phenomena Cadherin 5 Cadherins / genetics Cadherins / physiology* Fluorescence Resonance Energy Transfer Intercellular Junctions / physiology Mechanotransduction, Cellular / physiology Molecular Imaging Mutation Neovascularization, Physiologic / genetics Tensile Strength / physiology Zebrafish / embryology Zebrafish / genetics Zebrafish / physiology* Zebrafish Proteins / genetics Zebrafish Proteins / physiology* |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 17 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | UAS:RFP |