Reference - Detail
| RRC ID | 87180 |
|---|---|
| Author | Hayakawa M, Sakata A, Hayakawa H, Matsumoto H, Hiramoto T, Kashiwakura Y, Baatartsogt N, Fukushima N, Sakata Y, Suzuki-Inoue K, Ohmori T. |
| Title | Characterization and visualization of murine coagulation factor VIII-producing cells in vivo. |
| Journal | Sci Rep |
| Abstract |
Coagulation factors are produced from hepatocytes, whereas production of coagulation factor VIII (FVIII) from primary tissues and cell species is still controversial. Here, we tried to characterize primary FVIII-producing organ and cell species using genetically engineered mice, in which enhanced green fluorescent protein (EGFP) was expressed instead of the F8 gene. EGFP-positive FVIII-producing cells existed only in thin sinusoidal layer of the liver and characterized as CD31high, CD146high, and lymphatic vascular endothelial hyaluronan receptor 1 (Lyve1)+. EGFP-positive cells can be clearly distinguished from lymphatic endothelial cells in the expression profile of the podoplanin- and C-type lectin-like receptor-2 (CLEC-2)+. In embryogenesis, EGFP-positive cells began to emerge at E14.5 and subsequently increased according to liver maturation. Furthermore, plasma FVIII could be abolished by crossing F8 conditional deficient mice with Lyve1-Cre mice. In conclusion, in mice, FVIII is only produced from endothelial cells exhibiting CD31high, CD146high, Lyve1+, CLEC-2+, and podoplanin- in liver sinusoidal endothelial cells. |
| Volume | 11(1) |
| Pages | 14824 |
| Published | 2021-7-21 |
| DOI | 10.1038/s41598-021-94307-0 |
| PII | 10.1038/s41598-021-94307-0 |
| PMID | 34290295 |
| PMC | PMC8295325 |
| MeSH | Animals CD146 Antigen / metabolism Endothelial Cells / metabolism* Factor VIII / biosynthesis* Green Fluorescent Proteins / metabolism Immunohistochemistry Lectins, C-Type / metabolism Liver / cytology* Liver / embryology Membrane Glycoproteins / metabolism Membrane Transport Proteins Mice Platelet Endothelial Cell Adhesion Molecule-1 / metabolism Podoplanin Vesicular Transport Proteins / metabolism |
| IF | 3.998 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Mice | RBRC01828 RBRC04495 |