論文 - 詳細
| RRC ID | 87218 |
|---|---|
| 著者 | Amarbayasgalan S, Takahashi T, Sugiura Y, Shimizu K, Dorjsuren E, Luo L, Kamitani W. |
| タイトル | Bovine respiratory syncytial virus utilizes the human insulin-like growth factor 1 receptor in the late stages of infection. |
| ジャーナル | J Gen Virol |
| Abstract |
Bovine respiratory syncytial virus (BRSV) is a major viral pathogen associated with the bovine respiratory disease complex, which is a leading cause of morbidity, mortality and economic loss in the cattle industry worldwide. Clinical infection is most severe in young calves, where it commonly causes lower respiratory tract inflammation, bronchopneumonia and predisposition to secondary bacterial infections. In experimental research, BRSV is typically maintained in Vero and MDBK cells. Although reverse genetics systems have been established for BRSV, we developed a bacterial artificial chromosome-based reverse genetics system for the virus. We successfully recovered a recombinant BRSV with the ZsGreen reporter gene inserted between the P and M genes. The recombinant virus displayed comparable growth kinetics to the WT strain, demonstrating the utility of the system for generating reporter viruses. Reporter virus infectivity assessments in mammalian MDBK, VeroE6, HEp-2 and HEK293T cells revealed that HEK293T cells are permissive to BRSV. To investigate the potential role of human insulin-like growth factor 1 receptor (hIGF1R), which human RSV uses for entry, we infected insulin-like growth factor 1 receptor (IGF1R)-knockout (KO) 293 T cells with BRSV-ZsGreen. At 24 h post-infection (hpi), ZsGreen levels were similar between WT and hIGF1R-KO cells; however, by 72 hpi, viral spread was markedly reduced in hIGF1R-KO cells and correlated with IGF1R levels. These findings suggest that IGF1R is dispensable for early BRSV infection but contributes to efficient viral propagation in later stages. |
| 巻・号 | 107(3) |
| 公開日 | 2026-3-1 |
| DOI | 10.1099/jgv.0.002241 |
| PMID | 41880205 |
| PMC | PMC13016481 |
| MeSH | Animals Cattle Cattle Diseases* / virology Cell Line Chlorocebus aethiops HEK293 Cells Humans Receptor, IGF Type 1* / genetics Receptor, IGF Type 1* / metabolism Respiratory Syncytial Virus Infections* / metabolism Respiratory Syncytial Virus Infections* / veterinary Respiratory Syncytial Virus Infections* / virology Respiratory Syncytial Virus, Bovine* / genetics Respiratory Syncytial Virus, Bovine* / physiology Respiratory Syncytial Virus, Human Vero Cells Virus Replication |
| IF | 3.376 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.2 |
| リソース情報 | |
| ヒト・動物細胞 | BHK/T7-9(RCB4942) |