論文 - 詳細
| RRC ID | 87230 |
|---|---|
| 著者 | Nakatani S, Ueda H, Kawata A, Sato Y, Inomata K, Mano H, Wada M, Kobata K. |
| タイトル | Calcium-sensing receptor induces the apoptosis of chondrocytes in cooperation with phosphate transporter. |
| ジャーナル | FEBS Open Bio |
| Abstract |
Excess extracellular inorganic phosphate ions (Pi) and calcium ions (Ca2+) cause apoptosis and subsequent mineralization of chondrocytes. Here, we investigated the mechanism underlying the effect of these minerals. The chondrogenic cell line ATDC5 was treated with 2 mm Pi and/or Ca2+, and apoptosis, mineralization, and intracellular Pi concentrations were determined. Further, Pi- and Ca2+-treated cells were incubated with the Pi transporter (Pit-1) blocker phosphonoformic acid (PFA), the calcium-sensing receptor (CaSR) antagonist NPS-2143, and the CaSR agonist GdCl3. Individual addition of Pi and Ca2+ did not induce apoptosis and mineralization, while combined addition of the minerals induced both. The Pit-1 blocker and the CaSR antagonist completely inhibited the apoptosis induced by combined addition of Pi and Ca2+. Intracellular Pi concentration was remarkably increased by combined addition of Pi and Ca2+ as compared to the findings for individual addition. The Pit-1 blocker and CaSR antagonist completely inhibited the increase in intracellular Pi concentration induced by the combined addition of Pi and Ca2+. The CaSR agonist considerably increased the intracellular Pi concentration. Our results indicate that excess extracellular Ca2+ activates CaSR, which induces the intake of excess extracellular Pi through Pit-1 into ATDC5 cells. The resulting increase in intracellular Pi concentration induces apoptosis. |
| 巻・号 | 16(3) |
| ページ | 496-502 |
| 公開日 | 2026-3-1 |
| DOI | 10.1002/2211-5463.70142 |
| PMID | 41081312 |
| PMC | PMC12955750 |
| MeSH | Animals Apoptosis* / drug effects Apoptosis* / physiology Calcium / metabolism Cell Line Chondrocytes* / cytology Chondrocytes* / drug effects Chondrocytes* / metabolism Mice Phosphate Transport Proteins* / metabolism Phosphates / metabolism Receptors, Calcium-Sensing* / agonists Receptors, Calcium-Sensing* / antagonists & inhibitors Receptors, Calcium-Sensing* / metabolism |
| IF | 2.231 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ATDC5(RCB0565) |