| RRC ID |
87482
|
| Author |
Lin TI, Hsieh PY, Lin HJ, Chiang CK, Sheu JJ, Chang WT, Liau I, Hsu HY.
|
| Title |
Soy Protein-Cultured Mesenchymal Stem Cell-Secreted Extracellular Vesicles Target the Neurovascular Unit: Insights from a Zebrafish Brain Injury Model.
|
| Journal |
ACS Biomater Sci Eng
|
| Abstract |
Cerebral vascular disorders often accompany hypoxia-induced brain injury. In this study, we develop a zebrafish model of hypoxia-induced cerebral vascular injury to replicate the associated phenotypic changes, including cerebrovascular damage, neuronal apoptosis, and neurological dysfunction. We then explored the therapeutic potential of extracellular vesicles derived from Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) cultured on soy protein-coated surfaces. These vesicles demonstrated superior recovery efficacy, especially in restoring the blood-brain barrier integrity and improving neurological function. Our findings suggest that these potent therapeutic extracellular vesicles, easily produced from WJ-MSCs cultured in the presence of soy proteins, may mitigate hypoxia-induced brain injury by decreasing the severity of vascular disorder caused by oxidative stress. Protein-protein interactome analysis further suggests that multiple signaling pathways are likely involved in restoring normal neurovascular unit function.
|
| Volume |
11(3)
|
| Pages |
1432-1444
|
| Published |
2025-3-10
|
| DOI |
10.1021/acsbiomaterials.4c02304
|
| PMID |
40000145
|
| PMC |
PMC11897944
|
| MeSH |
Animals
Blood-Brain Barrier / metabolism
Brain Injuries* / metabolism
Brain Injuries* / pathology
Brain Injuries* / therapy
Cells, Cultured
Disease Models, Animal
Extracellular Vesicles* / chemistry
Extracellular Vesicles* / metabolism
Extracellular Vesicles* / transplantation
Mesenchymal Stem Cells* / cytology
Mesenchymal Stem Cells* / metabolism
Soybean Proteins* / chemistry
Soybean Proteins* / metabolism
Zebrafish
|
| IF |
4.152
|
| Resource |
| Zebrafish |
Tg(CM-isl1:GFP)rw0 |
