論文 - 詳細
| RRC ID | 87494 |
|---|---|
| 著者 | Farmer DT, Dukov JE, Chen HJ, Arata C, Hernandez-Trejo J, Xu P, Teng CS, Maxson RE, Crump JG. |
| タイトル | Cellular transitions during cranial suture establishment in zebrafish. |
| ジャーナル | Nat Commun |
| Abstract |
Cranial sutures separate neighboring skull bones and are sites of bone growth. A key question is how osteogenic activity is controlled to promote bone growth while preventing aberrant bone fusions during skull expansion. Using single-cell transcriptomics, lineage tracing, and mutant analysis in zebrafish, we uncover key developmental transitions regulating bone formation at sutures during skull expansion. In particular, we identify a subpopulation of mesenchyme cells in the mid-suture region that upregulate a suite of genes including BMP antagonists (e.g. grem1a) and pro-angiogenic factors. Lineage tracing with grem1a:nlsEOS reveals that this mid-suture subpopulation is largely non-osteogenic. Moreover, combinatorial mutation of BMP antagonists enriched in this mid-suture subpopulation results in increased BMP signaling in the suture, misregulated bone formation, and abnormal suture morphology. These data reveal establishment of a non-osteogenic mesenchyme population in the mid-suture region that restricts bone formation through local BMP antagonism, thus ensuring proper suture morphology. |
| 巻・号 | 15(1) |
| ページ | 6948 |
| 公開日 | 2024-8-13 |
| DOI | 10.1038/s41467-024-50780-5 |
| PII | 10.1038/s41467-024-50780-5 |
| PMID | 39138165 |
| PMC | PMC11322166 |
| MeSH | Animals Bone Morphogenetic Proteins* / genetics Bone Morphogenetic Proteins* / metabolism Cranial Sutures* / embryology Cranial Sutures* / growth & development Cranial Sutures* / metabolism Gene Expression Regulation, Developmental Mesoderm* / cytology Mesoderm* / embryology Mesoderm* / metabolism Mutation Osteogenesis* Signal Transduction Single-Cell Analysis Skull / embryology Zebrafish* / embryology Zebrafish* / genetics Zebrafish Proteins* / genetics Zebrafish Proteins* / metabolism |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 28 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(mmp9:egfp)tyt206 |