| RRC ID |
87494
|
| Author |
Farmer DT, Dukov JE, Chen HJ, Arata C, Hernandez-Trejo J, Xu P, Teng CS, Maxson RE, Crump JG.
|
| Title |
Cellular transitions during cranial suture establishment in zebrafish.
|
| Journal |
Nat Commun
|
| Abstract |
Cranial sutures separate neighboring skull bones and are sites of bone growth. A key question is how osteogenic activity is controlled to promote bone growth while preventing aberrant bone fusions during skull expansion. Using single-cell transcriptomics, lineage tracing, and mutant analysis in zebrafish, we uncover key developmental transitions regulating bone formation at sutures during skull expansion. In particular, we identify a subpopulation of mesenchyme cells in the mid-suture region that upregulate a suite of genes including BMP antagonists (e.g. grem1a) and pro-angiogenic factors. Lineage tracing with grem1a:nlsEOS reveals that this mid-suture subpopulation is largely non-osteogenic. Moreover, combinatorial mutation of BMP antagonists enriched in this mid-suture subpopulation results in increased BMP signaling in the suture, misregulated bone formation, and abnormal suture morphology. These data reveal establishment of a non-osteogenic mesenchyme population in the mid-suture region that restricts bone formation through local BMP antagonism, thus ensuring proper suture morphology.
|
| Volume |
15(1)
|
| Pages |
6948
|
| Published |
2024-8-13
|
| DOI |
10.1038/s41467-024-50780-5
|
| PII |
10.1038/s41467-024-50780-5
|
| PMID |
39138165
|
| PMC |
PMC11322166
|
| MeSH |
Animals
Bone Morphogenetic Proteins* / genetics
Bone Morphogenetic Proteins* / metabolism
Cranial Sutures* / embryology
Cranial Sutures* / growth & development
Cranial Sutures* / metabolism
Gene Expression Regulation, Developmental
Mesoderm* / cytology
Mesoderm* / embryology
Mesoderm* / metabolism
Mutation
Osteogenesis*
Signal Transduction
Single-Cell Analysis
Skull / embryology
Zebrafish* / embryology
Zebrafish* / genetics
Zebrafish Proteins* / genetics
Zebrafish Proteins* / metabolism
|
| Resource |
| Zebrafish |
Tg(mmp9:egfp)tyt206 |
