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RRC ID 87494
著者 Farmer DT, Dukov JE, Chen HJ, Arata C, Hernandez-Trejo J, Xu P, Teng CS, Maxson RE, Crump JG.
タイトル Cellular transitions during cranial suture establishment in zebrafish.
ジャーナル Nat Commun
Abstract Cranial sutures separate neighboring skull bones and are sites of bone growth. A key question is how osteogenic activity is controlled to promote bone growth while preventing aberrant bone fusions during skull expansion. Using single-cell transcriptomics, lineage tracing, and mutant analysis in zebrafish, we uncover key developmental transitions regulating bone formation at sutures during skull expansion. In particular, we identify a subpopulation of mesenchyme cells in the mid-suture region that upregulate a suite of genes including BMP antagonists (e.g. grem1a) and pro-angiogenic factors. Lineage tracing with grem1a:nlsEOS reveals that this mid-suture subpopulation is largely non-osteogenic. Moreover, combinatorial mutation of BMP antagonists enriched in this mid-suture subpopulation results in increased BMP signaling in the suture, misregulated bone formation, and abnormal suture morphology. These data reveal establishment of a non-osteogenic mesenchyme population in the mid-suture region that restricts bone formation through local BMP antagonism, thus ensuring proper suture morphology.
巻・号 15(1)
ページ 6948
公開日 2024-8-13
DOI 10.1038/s41467-024-50780-5
PII 10.1038/s41467-024-50780-5
PMID 39138165
PMC PMC11322166
MeSH Animals Bone Morphogenetic Proteins* / genetics Bone Morphogenetic Proteins* / metabolism Cranial Sutures* / embryology Cranial Sutures* / growth & development Cranial Sutures* / metabolism Gene Expression Regulation, Developmental Mesoderm* / cytology Mesoderm* / embryology Mesoderm* / metabolism Mutation Osteogenesis* Signal Transduction Single-Cell Analysis Skull / embryology Zebrafish* / embryology Zebrafish* / genetics Zebrafish Proteins* / genetics Zebrafish Proteins* / metabolism
リソース情報
ゼブラフィッシュ Tg(mmp9:egfp)tyt206