論文 - 詳細
| RRC ID | 87593 |
|---|---|
| 著者 | Vega-Badillo J, Zamore PD, Jouravleva K. |
| タイトル | Biochemical principles of miRNA targeting in flies. |
| ジャーナル | Nat Commun |
| Abstract |
MicroRNAs direct Argonaute proteins to repress complementary target mRNAs via mRNA degradation or translational inhibition. While mammalian miRNA targeting has been well studied, the principles by which Drosophila miRNAs bind their target RNAs remain to be fully characterized. Here, we use RNA Bind-n-Seq to systematically identify binding sites and measure their affinities for five highly expressed Drosophila miRNAs. Our results reveal a narrower range of binding site diversity in flies compared to mammals, with fly miRNAs favoring canonical seed-matched sites and exhibiting limited tolerance for imperfections within these sites. We also identified non-canonical site types, including nucleation-bulged and 3'-only sites, whose binding affinities are comparable to canonical sites. These findings establish a foundation for future computational models of Drosophila miRNA targeting, enabling predictions of regulatory outcomes in response to cellular signals, and advancing our understanding of miRNA-mediated regulation in flies. |
| 巻・号 | 17(1) |
| ページ | 1641 |
| 公開日 | 2026-1-20 |
| DOI | 10.1038/s41467-026-68360-0 |
| PII | 10.1038/s41467-026-68360-0 |
| PMID | 41559052 |
| PMC | PMC12905440 |
| MeSH | Animals Argonaute Proteins / genetics Argonaute Proteins / metabolism Binding Sites / genetics Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila melanogaster* / genetics Drosophila melanogaster* / metabolism Gene Expression Regulation MicroRNAs* / genetics MicroRNAs* / metabolism RNA, Messenger / genetics RNA, Messenger / metabolism |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 12 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#109027 |