Reference - Detail
| RRC ID | 87970 |
|---|---|
| Author | Schreier J, Pshanichnaya L, Kielisch F, Ketting RF. |
| Title | A genetic framework for RNAi inheritance in Caenorhabditis elegans. |
| Journal | EMBO Rep |
| Abstract |
Gene regulation by RNA interference (RNAi) is a conserved process driven by double-stranded RNA (dsRNA). It responds to exogenous cues and drives endogenous gene regulation. In Caenorhabditis elegans, RNAi can be inherited from parents to offspring. While a number of factors have been implicated in this inheritance process, we do not understand how and when they function. Using a new inheritance assay, we establish a hierarchy amongst previously identified inheritance factors. We show that the nuclear Argonaute protein HRDE-1 is required for RNAi establishment in parents and offspring, but not for the inheritance process. In contrast, the cytoplasmic Argonaute protein WAGO-3 is the only factor essential for inheritance, via sperm and oocyte, while not affecting establishment in either parent or offspring. We propose a cycle in which nuclear and cytoplasmic Argonaute proteins interact to generate both a silencing response and a cytoplasmic factor that transmits the silencing between parent and offspring, WAGO-3. Finally, we implicate the RNA helicase ZNFX-1 as a factor that allows the inherited WAGO-3 protein to trigger silencing in the offspring. |
| Volume | 26(16) |
| Pages | 4072-4099 |
| Published | 2025-8-1 |
| DOI | 10.1038/s44319-025-00512-7 |
| PII | 10.1038/s44319-025-00512-7 |
| PMID | 40624357 |
| PMC | PMC12373942 |
| MeSH | Animals Argonaute Proteins / genetics Argonaute Proteins / metabolism Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Male Oocytes / metabolism RNA Interference* RNA, Double-Stranded / genetics Spermatozoa / metabolism |
| Altmetric score |
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| The most frequently cited source | Bluesky |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm1019 tm1200 |