論文 - 詳細
| RRC ID | 87996 |
|---|---|
| 著者 | Attianese B, Wang H, Madsen K, Zeijdner M, Helin K, Abay-Nørgaard S, Salcini AE. |
| タイトル | Functional dissection of H3K4 methyltransferases reveals distinct catalytic and non-catalytic roles in C. elegans development. |
| ジャーナル | Development |
| Abstract |
The KMT2 class of histone methyltransferases regulates methylation of histone 3 lysine 4 (H3K4), a conserved post-translational modification associated with active transcription. However, previous studies have highlighted catalytic-independent functions of KMT2 members and questioned the influence of H3K4 methylation on gene expression. Here, we address this by generating catalytically inactive mutants of SET-2 and SET-16, the two KMT2 members in Caenorhabditis elegans. Through chromatin analysis, we determined the effect of SET-2 and SET-16 catalytic activities on H3K4me3 deposition and identified shared and distinct targets. Gene expression profiling showed that simultaneous inactivation of SET-2 and SET-16 catalytic activities results in gene deregulation independent of H3K4me3 status at transcription start sites. Finally, we examined the relevance of SET-2 and SET-16 catalytic activity on phenotypes identified in null mutants and found that SET-2 catalytic activity is essential for proper somatic development, whereas SET-16 enzymatic activity has cell type-specific roles. Interestingly, animals lacking SET-2 and SET-16 catalytic activity are viable and fertile under normal growth conditions. Our results reveal catalytic-dependent and -independent roles of KMT2 members, and that combined loss of SET-16 and SET-2 is compatible with life in C. elegans. |
| 巻・号 | 152(21) |
| 公開日 | 2025-11-1 |
| DOI | 10.1242/dev.204924 |
| PII | 369578 |
| PMID | 41122790 |
| PMC | PMC12633793 |
| MeSH | Animals Caenorhabditis elegans* / embryology Caenorhabditis elegans* / enzymology Caenorhabditis elegans* / genetics Caenorhabditis elegans* / growth & development Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Gene Expression Regulation, Developmental Histone-Lysine N-Methyltransferase* / genetics Histone-Lysine N-Methyltransferase* / metabolism Histones / metabolism Methylation Mutation Nuclear Proteins |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.2 |
| リソース情報 | |
| 線虫 | tm1630 |