論文 - 詳細
| RRC ID | 88052 |
|---|---|
| 著者 | Valenzuela-Villatoro M, Gómez-Orte E, Guerrero-Gómez D, Cheng Q, Zheleva A, Mora-Lorca JA, Petrovic D, O Neil NJ, Cerón J, Hatakeyama A, Onami S, Ordóñez-Luque A, Ayuso C, Askjaer P, Filipovic MR, Arnér ESJ, Cabello J, Miranda-Vizuete A. |
| タイトル | The transsulfuration pathway suppresses the embryonic lethal phenotype of glutathione reductase mutants in Caenorhabditis elegans. |
| ジャーナル | G3 (Bethesda) |
| Abstract |
The gsr-1 gene encodes the only glutathione reductase in Caenorhabditis elegans and gsr-1 loss-of-function alleles have a fully penetrant embryonic lethal phenotype. Therefore, maintenance of glutathione redox homeostasis is essential for nematode survival. We report here that impairment of the nonsense-mediated mRNA decay (NMD) pathway suppresses the embryonic lethality of gsr-1 mutants, allowing their normal development and growth. This NMD pathway dependent suppression requires cth-1 and cth-2 that encode 2 isoforms of cystathionine-γ-lyase that catalyze the conversion of cystathionine to cysteine through the transsulfuration pathway. In contrast, the thioredoxin system that can also provide cysteine through the cystine reduction pathway appears to be dispensable for the suppression of the lethal phenotype of gsr-1 embryos when the NMD pathway is inactivated. Together, our data indicate that increasing the activity of the reverse transsulfuration pathway can compensate the detrimental effect of the gsr-1 mutation, raising the interesting question of why C. elegans has not preserved such compensatory mechanism to avoid the embryonic lethality of these mutants. |
| 巻・号 | 15(8) |
| 公開日 | 2025-8-6 |
| DOI | 10.1093/g3journal/jkaf102 |
| PII | 8126572 |
| PMID | 40333285 |
| PMC | PMC12341920 |
| MeSH | Animals Caenorhabditis elegans* / embryology Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Embryo, Nonmammalian / metabolism Genes, Lethal Glutathione Reductase* / genetics Glutathione Reductase* / metabolism Mutation* Nonsense Mediated mRNA Decay Phenotype |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3574 tm5906 tm5719 tm2462 |