| RRC ID |
88073
|
| Author |
Dai W, Zhai Y, Yang F, Chen W, Liu C, Tian Y, Huang F, Cai M, Zheng L, Cheng W, Chen W, Zhang J.
|
| Title |
Different concentrations of 2-Undecanone triggers repellent and nematicidal responses in Caenorhabditis elegans.
|
| Journal |
Sci Rep
|
| Abstract |
The compound 2-undecanone is widely distributed in the natural environment and exhibits a dual-action mechanism against nematodes. It demonstrates repellency and contact toxicity against both Caenorhabditis elegans and Meloidogyne incognita. However, research on the dual-function mechanism of 2-undecanone remains relatively limited. In this study, using chemotaxis experiments, we found that 2-undecanone (at concentrations of 1-5 mg/mL) signal is detected through AWB olfactory sensory neurons in nematode, and then transduced through the cGMP pathway to induce repellent behavior. Moreover, we observed that 2-undecanone (at concentrations of 0.06-0.08 mg/mL) induces intracellular calcium accumulation and causes lysosomal membrane rupture. We further identified Hsp70 A and V-ATPase A as the targets of 2-undecanone responsible for its contact killing effect. Furthermore, 2-undecanone was found to alter sphingomyelin metabolism in both wild-type C. elegans and hsp-1 mutants. This alteration led to decreased acid sphingomyelinase activity, reduced ceramide levels, and increased sphingomyelin levels. These results indicated that 2-undecanone has dual functions and two target receptors Hsp70 A and V-ATPase A against nematodes, and one AWB olfactory neuron repelled nematodes. The dual-action mode of 2-undecanone can decrease the nematodes' tolerance to the compound appropriately, extending its efficacy duration. Understanding the dual function mechanism of action of 2-undecanone will aid in devising innovative approaches for managing nematode control.
|
| Volume |
15(1)
|
| Pages |
14186
|
| Published |
2025-4-23
|
| DOI |
10.1038/s41598-025-95332-z
|
| PII |
10.1038/s41598-025-95332-z
|
| PMID |
40269070
|
| PMC |
PMC12019385
|
| MeSH |
Animals
Antinematodal Agents* / pharmacology
Caenorhabditis elegans* / drug effects
Caenorhabditis elegans* / metabolism
Caenorhabditis elegans* / physiology
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism
Calcium / metabolism
Chemotaxis / drug effects
Cyclic GMP / metabolism
HSP70 Heat-Shock Proteins / genetics
HSP70 Heat-Shock Proteins / metabolism
Ketones* / pharmacology
Olfactory Receptor Neurons / drug effects
Olfactory Receptor Neurons / metabolism
Signal Transduction / drug effects
Vacuolar Proton-Translocating ATPases / metabolism
|
| Resource |
| C.elegans |
tm5076
tm4559 |
