Reference - Detail
| RRC ID | 88113 |
|---|---|
| Author | Kirshner JA, Picard CL, Weiser NE, Mehta N, Feng S, Murphy VN, Vakhnovetsky A, Alessi AF, Xiao C, Inoki K, El Mouridi S, Frøkjær-Jensen C, Jacobsen SE, Kim JK. |
| Title | Regulation of MORC-1 is key to the CSR-1-mediated germline gene licensing mechanism in C. elegans. |
| Journal | Sci Adv |
| Abstract |
The Argonaute CSR-1 is essential for germline development in C. elegans. Loss of CSR-1 leads to the down-regulation of thousands of germline-expressed genes, supporting a model in which CSR-1 "licenses" gene expression via a poorly understood mechanism. In contrast, a small subset of genes is up-regulated in csr-1 mutants, including morc-1, which encodes a conserved GHKL-type ATPase. We show that morc-1 is overexpressed in csr-1 mutants and accumulates over CSR-1 licensed targets, coinciding with aberrant gain of H3K9me3, reduced H3K36me3, and transcriptional repression. Notably, loss of morc-1 fully rescues these chromatin defects and partially restores gene expression and fertility in csr-1 mutants. Conversely, ectopic overexpression of MORC-1 in the wild-type germ line is sufficient to repress CSR-1 licensed targets and severely compromise fertility. These findings support a model in which CSR-1 prevents MORC-1 overexpression and consequent misregulation of CSR-1 licensed genes. |
| Volume | 11(25) |
| Pages | eado4170 |
| Published | 2025-6-20 |
| DOI | 10.1126/sciadv.ado4170 |
| PMID | 40540580 |
| PMC | PMC12180489 |
| MeSH | Adenosine Triphosphatases* / genetics Adenosine Triphosphatases* / metabolism Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Chromatin / genetics Chromatin / metabolism Fertility / genetics Gene Expression Regulation Gene Expression Regulation, Developmental Germ Cells* / metabolism Histones / metabolism Mutation |
| Altmetric score |
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| The most frequently cited source | Bluesky |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm6048 tm892 |