Reference - Detail
| RRC ID | 88483 |
|---|---|
| Author | Kato H, Uratsuji I, Nakadai Y, Ochiai W. |
| Title | Protective Effects of the Phytocannabinoid Cannabidiol on Disuse-Induced Muscle Atrophy through Modulation of Proteolysis and Mitochondrial Regulation. |
| Journal | Biol Pharm Bull |
| Abstract |
Muscle atrophy induced by prolonged inactivity (disuse), including denervation-induced atrophy, is accompanied by oxidative stress, inflammation, and dysregulated protein turnover, yet no effective pharmacological therapy is currently available. Cannabidiol (CBD), a non-psychoactive phytocannabinoid derived from Cannabis sativa, has been reported to exhibit anti-inflammatory and antioxidant properties; however, its potential involvement in disuse-related muscle atrophy has not been fully characterized. In this study, to evaluate the potential effects of CBD on disuse-related muscle atrophy, we employed both in vivo and in vitro models. A mouse model of sciatic nerve resection-induced muscle atrophy was used for the in vivo experiments, while C2C12 myotubes were utilized for the in vitro analyses. In the denervated mouse model, CBD attenuated the decrease in muscle mass in the tibialis anterior and gastrocnemius muscles, as well as the decline in treadmill running performance. CBD also reduced oxidative stress and suppressed the denervation-induced upregulation of Atrogin-1 and muscle RING-finger 1 (MuRF1) proteins, as well as tumor necrosis factor-α (TNF-α) mRNA. Furthermore, CBD partially restored the decreased mitochondrial markers observed following denervation. In vitro, CBD similarly suppressed MuRF1 and Atrogin-1 protein levels and TNF-α mRNA expression in C2C12 myotubes. These findings suggest that CBD is associated with protective effects against disuse-related muscle atrophy, accompanied by reductions in oxidative stress markers, alterations in proteolytic pathways, and changes in mitochondrial-related markers. This study highlights a previously underexplored biological effect of a natural phytocannabinoid and supports further investigation of CBD as a potential supportive strategy for disuse-related muscle wasting. |
| Volume | 49(5) |
| Pages | 822-831 |
| Published | 2026-1-1 |
| DOI | 10.1248/bpb.b26-00020 |
| PMID | 42161484 |
| MeSH | Animals Cannabidiol* / pharmacology Cannabidiol* / therapeutic use Cell Line Male Mice Mice, Inbred C57BL Mitochondria* / drug effects Mitochondria* / metabolism Muscle Fibers, Skeletal / drug effects Muscle Fibers, Skeletal / metabolism Muscle Proteins / metabolism Muscle, Skeletal / drug effects Muscle, Skeletal / innervation Muscle, Skeletal / metabolism Muscle, Skeletal / pathology Muscular Atrophy* / drug therapy Muscular Atrophy* / metabolism Muscular Atrophy* / pathology Oxidative Stress / drug effects Proteolysis / drug effects SKP Cullin F-Box Protein Ligases / metabolism Tripartite Motif Proteins / metabolism Tumor Necrosis Factor-alpha / genetics Tumor Necrosis Factor-alpha / metabolism Ubiquitin-Protein Ligases / metabolism |
| IF | 1.863 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.7 |
| Resource | |
| Human and Animal Cells | C2C12(RCB0987) |