RRC ID 888
Author Saito TT, Tougan T, Kasama T, Okuzaki D, Nojima H.
Title Mcp7, a meiosis-specific coiled-coil protein of fission yeast, associates with Meu13 and is required for meiotic recombination.
Journal Nucleic Acids Res.
Abstract We previously showed that Meu13 of Schizosaccharomyces pombe functions in homologous pairing and recombination at meiosis I. Here we show that a meiosis-specific gene encodes a coiled-coil protein that complexes with Meu13 during meiosis in vivo. This gene denoted as mcp7+ (after meiotic coiled-coil protein) is an ortholog of Mnd1 of Saccharomyces cerevisiae. Mcp7 proteins are detected on meiotic chromatin. The phenotypes of mcp7Delta cells are similar to those of meu13Delta cells as they show reduced recombination rates and spore viability and produce spores with abnormal morphology. However, a delay in initiation of meiosis I chromosome segregation of mcp7Delta cells is not so conspicuous as meu13Delta cells, and no meiotic delay is observed in mcp7Deltameu13Delta cells. Mcp7 and Meu13 proteins depend on each other differently; Mcp7 becomes more stable in meu13Delta cells, whereas Meu13 becomes less stable in mcp7Delta cells. Genetic analysis shows that Mcp7 acts in the downstream of Dmc1, homologs of Escherichia coli RecA protein, for both recombination and subsequent sporulation. Taken together, we conclude that Mcp7 associates with Meu13 and together they play a key role in meiotic recombination.
Volume 32(11)
Pages 3325-39
Published 2004
DOI 10.1093/nar/gkh654
PII 32/11/3325
PMID 15210864
PMC PMC443530
MeSH Amino Acid Sequence CDC2 Protein Kinase / metabolism Carrier Proteins / genetics Carrier Proteins / metabolism Carrier Proteins / physiology* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Cell Nucleus / chemistry Chromosome Segregation Conserved Sequence DNA-Binding Proteins / metabolism Gene Deletion Meiosis Molecular Sequence Data Recombination, Genetic* Saccharomyces cerevisiae Proteins Schizosaccharomyces / cytology Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Signal Transduction Spores, Fungal / cytology
IF 11.561
Times Cited 21
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Yeast CD16-1 FY9906(TP4-1D) FY9907(TP4-5A) FY16751(ST95) FY16676(NP29-5B) FY16667(NP24-1D) FY7051(JZ670) FY16550(MS101-4)