| RRC ID |
888
|
| 著者 |
Saito TT, Tougan T, Kasama T, Okuzaki D, Nojima H.
|
| タイトル |
Mcp7, a meiosis-specific coiled-coil protein of fission yeast, associates with Meu13 and is required for meiotic recombination.
|
| ジャーナル |
Nucleic Acids Res
|
| Abstract |
We previously showed that Meu13 of Schizosaccharomyces pombe functions in homologous pairing and recombination at meiosis I. Here we show that a meiosis-specific gene encodes a coiled-coil protein that complexes with Meu13 during meiosis in vivo. This gene denoted as mcp7+ (after meiotic coiled-coil protein) is an ortholog of Mnd1 of Saccharomyces cerevisiae. Mcp7 proteins are detected on meiotic chromatin. The phenotypes of mcp7Delta cells are similar to those of meu13Delta cells as they show reduced recombination rates and spore viability and produce spores with abnormal morphology. However, a delay in initiation of meiosis I chromosome segregation of mcp7Delta cells is not so conspicuous as meu13Delta cells, and no meiotic delay is observed in mcp7Deltameu13Delta cells. Mcp7 and Meu13 proteins depend on each other differently; Mcp7 becomes more stable in meu13Delta cells, whereas Meu13 becomes less stable in mcp7Delta cells. Genetic analysis shows that Mcp7 acts in the downstream of Dmc1, homologs of Escherichia coli RecA protein, for both recombination and subsequent sporulation. Taken together, we conclude that Mcp7 associates with Meu13 and together they play a key role in meiotic recombination.
|
| 巻・号 |
32(11)
|
| ページ |
3325-39
|
| 公開日 |
2004-1-1
|
| DOI |
10.1093/nar/gkh654
|
| PII |
32/11/3325
|
| PMID |
15210864
|
| PMC |
PMC443530
|
| MeSH |
Amino Acid Sequence
CDC2 Protein Kinase / metabolism
Carrier Proteins / genetics
Carrier Proteins / metabolism
Carrier Proteins / physiology*
Cell Cycle Proteins / genetics
Cell Cycle Proteins / metabolism*
Cell Nucleus / chemistry
Chromosome Segregation
Conserved Sequence
DNA-Binding Proteins / metabolism
Gene Deletion
Meiosis
Molecular Sequence Data
Recombination, Genetic*
Saccharomyces cerevisiae Proteins
Schizosaccharomyces / cytology
Schizosaccharomyces / genetics*
Schizosaccharomyces / metabolism
Schizosaccharomyces pombe Proteins / genetics
Schizosaccharomyces pombe Proteins / metabolism*
Signal Transduction
Spores, Fungal / cytology
|
| IF |
11.502
|
| 引用数 |
24
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 酵母 |
CD16-1
FY9906(TP4-1D)
FY9907(TP4-5A)
FY16751(ST95)
FY16676(NP29-5B)
FY16667(NP24-1D)
FY7051(JZ670)
FY16550(MS101-4) |
