| Abstract |
Two obligately anaerobic, Gram-stain-negative, non-spore-forming, rod-shaped, motile and mesophilic bacteria, designated as strains AN17-1T and AN17-2T, were isolated from deep-marine sediments collected from the western Pacific Ocean. The phylogenetic analysis based on the 16S rRNA gene with the most closely related species showed 98.5 and 98.8% similarities of strains AN17-1T and AN17-2T with Vallitalea guaymasensis Ra1766G1T, respectively. The genome sizes and G+C contents of strains AN17-1T and AN17-2T were 6.0 Mb and 30.3 mol% and 5.4 Mb and 30.9 mol%, respectively. The genome-to-genome distance and the average nucleotide identity values between strains AN17-1T and AN17-2T and V. guaymasensis Ra1766G1T were lower than the thresholds for species delimitation. Elemental sulphur, sulphate, thiosulphate, sulphite, fumarate, nitrate and nitrite were not used as terminal electron acceptors by strains AN17-1T and AN17-2T. The most abundant fatty acids of strains AN17-1T and AN17-2T were iso-C15:0 and C16:0, respectively. The commonly detected polar lipids of both strains were diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid, glycolipid, two unidentified phospholipids and one unidentified polar lipid. The results suggest that strains AN17-1T and AN17-2T represent novel species of the genus Vallitalea. Therefore, the new names, Vallitalea sediminicola sp. nov. and Vallitalea maricola sp. nov., are proposed for strains AN17-1T (=NBRC 115486T=DSM 116091T) and AN17-2T (=NBRC 115487T=DSM 116092T) as the type strains, respectively.
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