論文 - 詳細
| RRC ID | 88849 |
|---|---|
| 著者 | Kang MS, Yu JY, Kim YH, Dong K, Srinivasan S, Lee SS. |
| タイトル | Nocardioides eburneus sp. nov., isolated from commercial soil. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A novel bacterial strain, designated DS6T, was isolated from the commercial soil for biofertilization and characterized as a novel species within the Actinomycetota phylum. The morphological and biochemical analysis revealed that strain DS6T is a Gram-positive, rod-shaped bacterium with pili, forming ivory colonies on R2A agar after 3 days of incubation at 25 °C. The strain shows a broad growth pH range of 4-9, with an optimum pH of 8, and can tolerate temperatures between 20 and 45 °C, optimally growing at 37 °C. Salinity tolerance ranges from 0% to 5%, with optimal growth occurring at 0-1%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain DS6T is closely related to Nocardioides marinus KCCM 4231T (96.4%), Nocardioides panaciterrulae KCTC 19471T (96.3%), Nocardioides gansuensis KCTC 49117T (96.3%) and Nocardioides ginkgobilobae KCTC 39594T (96.2%). Biochemical tests showed that the strain is oxidase negative and catalase positive. The major fatty acids were C16 : 0 iso (26.85%) and C18 : 0 10-methyl (15.9%). The major respiratory quinone was MK-8 (H4) and the strain contained ll-2,6-diaminopimelic acid as the cell-wall diamino acid. The major polar lipids consist of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phospholipids, phosphatidylinositolmannosides and unknown lipids. The DNA G+C mol% of genomic DNA is 71.3. Based on its distinct phylogenetic, phenotypic and biochemical characteristics, strain DS6T is proposed to represent a novel species of the genus Nocardioides, for which the name Nocardioides eburneus sp. nov. is proposed. The type strain is DS6T (KEMB 22008T=KCTC 59264T=JCM 37205T). |
| 巻・号 | 75(4) |
| 公開日 | 2025-4-1 |
| DOI | 10.1099/ijsem.0.006743 |
| PMID | 40237765 |
| MeSH | Actinomycetales* / classification Actinomycetales* / genetics Actinomycetales* / isolation & purification Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics Diaminopimelic Acid / analysis Diaminopimelic Acid / chemistry Fatty Acids / analysis Fatty Acids / chemistry Phospholipids / analysis Phospholipids / chemistry Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Soil Microbiology* Vitamin K 2 / analogs & derivatives Vitamin K 2 / chemistry |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Blogs |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM37205 |