| 著者 |
Ma J, Hou Q, Zhou H, Yang J, Yang C, Liu J, Wang Y, Cao L, Pu J, Wang W, Jin D, Lu S, Xu J.
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| Abstract |
Four novel, Gram-stain-positive, catalase-positive, oxidase-negative, aerobic and non-motile strains, designated MJ206T, MJ278, MJ170T and MJ173, were isolated from the faeces of Cygnus atratus in the Qinghai-Tibet Plateau of China. A comparative analysis of 16S rRNA gene sequences revealed that strain pairs MJ206T and MJ278, as well as MJ170T and MJ173, belong to the genus Georgenia. Strain pair MJ206T/MJ278 is most closely related to 'Georgenia faecalis' ZLJ0423 (98.19% 16S rRNA gene sequence similarity), while the strain pair MJ170T/MJ173 is most related to Georgenia satyanarayanai JC82T (98.00% 16S rRNA gene sequence similarity). These relationships were further confirmed by phylogenetic analyses based on the 16S rRNA gene and genome sequences. The two type strains (MJ206T and MJ170T) have low digital DNA-DNA hybridization values (20.3-23.9%) and average nt identity values (75.3-80.9%) with all available genomes in the genus Georgenia. The DNA G+C contents of strains MJ206T and MJ170T were 73.5 and 72.0 mol%, respectively. The predominant cellular fatty acids of both strains (MJ206T and MJ170T) were anteiso-C15 : 0 and anteiso-C15:1 A, but strain MJ206T has an additional major fatty acid, which is anteiso-C16 : 0. MK-8 (H4) was the major menaquinone. The major polar lipids detected in strains MJ206T and MJ170T included diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The cell wall aa detected in the two novel species were alanine (Ala), glutamic acid (Glu), glycine (Gly), aspartic acid (Asp) and ornithine acid (Orn); the shared whole cell wall sugars consisted of ribose (Rib), glucose (Glu) and galactose (Gal). All genotypic and phenotypic data indicated that these four strains represented two novel species in the genus Georgenia, for which the names Georgenia wangjunii sp. nov. and Georgenia sunbinii sp. nov. are proposed, respectively. The type strains are MJ206T (=CGMCC 1.4699T=JCM 37421T) and MJ170T (=CGMCC 1.4698T=JCM 37420T).
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