論文 - 詳細
| RRC ID | 88857 |
|---|---|
| 著者 | Cheng G, Schnürer A, Westerholm M. |
| タイトル | Microaceticoccus formicicus gen. nov., sp. nov., an ammonia-tolerant formate-utilizing bacterium originating from a biogas process. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A strictly anaerobic bacterial strain, designated as AMB_02T, was isolated from a propionate enrichment culture obtained from a high-ammonia biogas digester. The cells were anaerobic and coccoid (0.5 µm), often appearing as diplococci or in a short chain of three to four cells. Growth was observed between 20 and 45 °C (optimum at 37-39 °C), with an initial pH of 6.5-9.0 (optimum pH 8.0-8.5), and the species tolerated up to 0.8 M NH4Cl and 0.5 M NaCl. The major cellular fatty acids were C16 : 0 (31.6%), C14 : 0 (14.6%) and C18 : 0 (13.3%). AMB_02T grew with formate, carbohydrates and aa, including asparagine, histidine, tryptone and tryptophan. Acetate was the major product formed. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain AMB_02T was most closely related to the species Citroniella saccharovorans (92.5%). The genome of strain AMB_02T was 2.5 Mb in length with a G+C content of 34.8 mol%, and 2,354 protein-coding genes were predicted. Furthermore, genes coding for the reductive glycine pathway potentially used for formate metabolism were identified. Comparative genomic analysis of AMB_02T revealed the closest similarity to C. saccharovorans [21.2% digital DNA-DNA hybridization (dDDH) and 77.4% average nt identity (ANI)] and to Parvimonas micra (24.4% dDDH and 76.9% ANI). Based on the phenotypic characteristics and phylogenetic analyses, AMB_02T is regarded as a novel genus, Microaceticoccus, within the family Peptoniphilaceae for which the species name Microaceticoccus formicicus is proposed. The type strain is AMB_02T=DSM 110248T=JCM 39108T. |
| 巻・号 | 75(5) |
| 公開日 | 2025-5-1 |
| DOI | 10.1099/ijsem.0.006773 |
| PMID | 40338641 |
| PMC | PMC12079837 |
| MeSH | Ammonia* / metabolism Bacterial Typing Techniques Base Composition Biofuels* / microbiology DNA, Bacterial / genetics Fatty Acids / analysis Fatty Acids / chemistry Formates* / metabolism Genome, Bacterial Micrococcaceae* / classification Micrococcaceae* / genetics Micrococcaceae* / isolation & purification Micrococcaceae* / metabolism Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM39108 |