| RRC ID |
88858
|
| 著者 |
Kang Z, Lei W, Pu J, Zhao L, Min G, Liu Y, Chang W, Gao Y, Dong K, Sun B.
|
| タイトル |
Identification of Roseomonas xinghualingensis sp. nov. isolated from hospital air.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
The genus Roseomonas comprises Gram-negative bacteria widely distributed in the environment and can cause human infections. However, the genomic characteristics and pathogenicity of Roseomonas remain poorly studied. Two new Gram-negative, mesothermic, aerobic and coccoid strains (SXEYE001T and SXEYE002) were isolated from hospital air. Colonies grown on R2A plates at 28 °C for 3 days were round with neat, raised edges and a pale pink colour. Sequencing of the 16S rRNA gene revealed that the two strains are 98.4% similar to 'Roseomonas chloroacetimidivorans' BUT 13T. The 16S rRNA gene sequence was observed to be 97.4% similar to Roseomonas pecuniae N75T. Genome-based phylogeny demonstrated that Roseomonas harenae CPCC 101081T and 'R. chloroacetimidivorans' BUT-13Tspecies are close relatives to strains SXEYE001T and SXEYE002. The average nucleotide identity between the strains and species of the genus Roseomonas was <95.0%. The G+C contents of strains SXEYE001T and SXEYE002 were calculated to be 67.8mol% and 67.9mol%, respectively. The major polar lipids were determined to be diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine, with coenzyme Q-10 serving as the primary respiratory quinone. Based on comprehensive genotypic, phenotypic, phylogenetic and phylogenomic analyses, we concluded that strains SXEYE001T and SXEYE002 were indicative of a novel species within the genus Roseomonas, for which the name Roseomonas xinghualingensis sp. nov. is proposed, with SXEYE001T (=GDMCC 1.3626T=JCM 35902T) as the type strain.
|
| 巻・号 |
75(5)
|
| 公開日 |
2025-5-1
|
| DOI |
10.1099/ijsem.0.006775
|
| PMID |
40338647
|
| MeSH |
Air Microbiology*
Bacterial Typing Techniques
Base Composition
DNA, Bacterial / genetics
Fatty Acids / chemistry
Genome, Bacterial
Hospitals
Methylobacteriaceae* / classification
Methylobacteriaceae* / genetics
Methylobacteriaceae* / isolation & purification
Phospholipids / analysis
Phospholipids / chemistry
Phylogeny*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Ubiquinone / analogs & derivatives
Ubiquinone / chemistry
|
| リソース情報 |
| 一般微生物 |
JCM35902 |
