| RRC ID |
88876
|
| Author |
Gao F, Zhang M, Yang Q, Zhao L, Xie J, Yang C, Du X, Kang Z, Pu J, Zheng H, Xu J, Dong K.
|
| Title |
Brachymonas wangyanguii sp. nov., isolated from the coal samples in Shanxi Province of China.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
Two Gram-negative, oxidase-positive, catalase-positive, non-spore-forming, motile, short-rod strains (G13T and G34) were isolated from in situ coal block samples. Phylogenetic analyses based on 16S rRNA genes and 718 core genes indicated that both strains belonged to the genus Brachymonas, with the closest relative being Brachymonas chironomi AIMA4T as their closest relative. Average nucleotide identity and digital DNA-DNA hybridization values were 79.4-89.27% and 23.6-37.0% below the species-level thresholds. Further chemotaxonomic analysis revealed that both strains possessed C16:1ω7c and/or C16:1ω6c, C18:1ω7c and C16:0 as major cellular fatty acids, with Q-8 as the predominant respiratory quinone. The polar lipid profile was dominated by diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Phenotypic and phylogenetic evidence support the proposal that strain G13T represents a novel species of the genus Brachymonas, with the name Brachymonas wangyanguii sp. nov. The type strain is G13T (=GDMCC 1.4691T=JCM 37746T).
|
| Volume |
75(7)
|
| Published |
2025-7-1
|
| DOI |
10.1099/ijsem.0.006837
|
| PMID |
40632584
|
| MeSH |
Bacterial Typing Techniques
Base Composition
China
Coal* / microbiology
DNA, Bacterial / genetics
Fatty Acids / analysis
Fatty Acids / chemistry
Nucleic Acid Hybridization
Phospholipids / analysis
Phylogeny*
Quinones / analysis
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
|
| Resource |
| General Microbes |
JCM37746 |
