| Abstract |
A Gram-stain-negative, aerobic bacterium, designated W635T, was isolated from saline lake water collected from Lake Ayaqqum in Xinjiang province, China. Cells were catalase and oxidase positive, rod-shaped and non-motile. Growth occurred at 20-37 °C (optimum 28-30 °C), pH 7.0-9.5 (optimum 7.5-8.0) and with 0.5-16.0% NaCl (optimum 2.0-4.0%, w/v). Chemotaxonomic analysis revealed that the sole respiratory quinone was ubiquinone-10. The major fatty acids included C16:0, C19:0 cyclo ω8c, C18:1 ω7c 11-methyl and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c). The major polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one uncharacterized phospholipid and four uncharacterized lipids. Sequence similarity analysis of the 16S rRNA gene revealed that strain W635T presented the highest similarity to Roseovarius nitratireducens TFZT (95.47%), followed by members of the genus Nioella (95.11-95.25%). Phylogenetic analysis based on multilocus sequence analysis and whole-genome sequencing indicated that strain W635T is most closely related to the genus Nioella. The genomic DNA G+C content of the strain W635T was 65.8 mol%. The average amino acid identity values between strain W635T and Nioella nitratireducens SSW136T, Nioella sediminis JS7-11T, Nioella aestuarii JCM30752T, and Nioella ostreopsis Z7-4T were 79.02%, 80.04%, 79.77% and 80.05%, respectively. The average nucleotide identity and in silico DNA-DNA hybridization values between strain W635T and these strains were 78.37-79.78% and 19.8-20.80%, respectively. Based on phenotypic, chemotaxonomic, genotypic and genomic characteristics, strain W635T represents a novel species of the genus Nioella, for which the name Nioella halotolerans is proposed. The type strain is W635T (=KCTC 62429T =MCCC 1K03491T).
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