| RRC ID |
88882
|
| 著者 |
Lange-Enyedi NT, Tóth E, Abbaszade G, Németh P, Garvie LAJ, Wolf J, Neumann-Schaal M, Khayer B, Sipos G, Makk J.
|
| タイトル |
Pseudogemmobacter sonorensis sp. nov., a new alphaproteobacterium isolated from the slime flux of a tree (Populus fremontii) in the Sonoran Desert (Arizona, USA).
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
The bacterial strain PA1-206BT is a Gram-stain-negative, aerobic, non-motile and non-spore-forming bacterium with an irregular rod shape. It was isolated from a tree wound exudate of the Populus fremontii trunk in the Sonoran Desert (USA), and its taxonomic position was investigated by a polyphasic approach. Strain PA1-206BT grew optimally at 28-30 °C and from pH 6 to 10 without NaCl. Based on 16S rRNA gene sequence analysis, this isolate showed only 96.0% sequence similarity to the type strain of Pseudogemmobacter hezensis and similarity of 94.4-95.7% to other species of the genus. Phylogenetic analysis based on whole-proteome comparisons placed strain PA1-206BT within the genus Pseudogemmobacter but, on a distinct branch, clearly separated from its closest relatives. The major isoprenoid quinone of the strain was ubiquinone Q-10. The predominant fatty acids (>5%) were C18:1 ω7c, C16:0 and 11-methyl-C18:1 ω7c. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid and two unidentified phospholipids were present. The assembled draft genome of strain PA1-206BT had 115 contigs with a total length of 4.5 Mb and a G+C content of 67.4 mol%. The overall genome-related indices (average nucleotide identity <80.4%, average amino acid identity <70.6%, digital DNA-DNA hybridization <21.6%) with respect to close relatives were below the corresponding threshold to demarcate bacterial species. Strain PA1-206BT (=DSM 115559T=NCAIM B.02680T) is suggested as the type strain of a novel Pseudogemmobacter species, for which the name Pseudogemmobacter sonorensis sp. nov. is proposed.
|
| 巻・号 |
75(7)
|
| 公開日 |
2025-7-1
|
| DOI |
10.1099/ijsem.0.006859
|
| PMID |
40728944
|
| MeSH |
Bacterial Typing Techniques
Base Composition
DNA, Bacterial / genetics
Desert Climate
Fatty Acids / analysis
Fatty Acids / chemistry
Methylobacteriaceae* / classification
Methylobacteriaceae* / genetics
Methylobacteriaceae* / isolation & purification
Nucleic Acid Hybridization
Phospholipids / analysis
Phylogeny*
Populus* / microbiology
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Soil Microbiology*
Trees / microbiology
Ubiquinone / analogs & derivatives
Ubiquinone / analysis
Ubiquinone / chemistry
|
| リソース情報 |
| 一般微生物 |
JCM17277 |
