| 著者 |
Yang S, Li J, Rong X, Chen Q, Lu X, Zhang J, Deng MR, Zhu H, Feng GD.
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| Abstract |
Two yellow-pigmented, Gram-stain-positive and aerobic bacterial strains, designated RRHDQ10T and RRHDQ66T, were isolated from citrus leaves collected from a well-known production area located in Renhua County of Guangdong Province, P. R. China. The 16S rRNA gene comparison showed that strain RRHDQ10T shared the highest similarity to Curtobacterium ammoniigenes NBRC 101786T (98.32%) and Curtobacterium albidum DSM 20512T (98.32%), and strain RRHDQ66T shared that to Curtobacterium flaccumfaciens JCM 9670T (99.86%) and Curtobacterium allii 20TX0166T (99.86%). Phylogenetic analyses based on the 16S rRNA gene and genome sequences showed that they belonged to the genus Curtobacterium, among which strain RRHDQ10T was most closely related to C. ammoniigenes NBRC 101786T, and strain RRHDQ66T showed a close relationship with C. flaccumfaciens JCM 9670T and Curtobacterium herbarum NBRC 103064T. The genome-derived average nucleotide identity between the two strains and their closely related type strains was below 82.06% and 85.55%, respectively, and the corresponding digital DNA-DNA hybridization values were below 22.3% and 26.2%, respectively. Anteiso-C15:0 and iso-C16: 0 were their major fatty acids. Moreover, summed feature 8 (C18:1 ω7c and/or C18:1 ω6c) and anteiso-C17 : 0 served as major components in strains RRHDQ10T and RRHDQ66T, respectively. They took menaquinone-9 (MK-9) as the predominant respiratory quinone and ornithine as the principal cell-wall diamino acid. Their major polar lipids were phosphatidylglycerol, diphosphatidylglycerol and three unidentified glycolipids. The genomic DNA G+C contents of strains RRHDQ10T and RRHDQ66T were 71.2 and 70.5 mol%, respectively. Based on phenotypic, genotypic and chemotaxonomic analyses, they should be considered as two novel species of the genus Curtobacterium, for which the names Curtobacterium phyllosphaerae sp. nov. and Curtobacterium guangdongense sp. nov. are proposed, with RRHDQ10T (=GDMCC 1.2671T=JCM 34831T) and RRHDQ66T (=GDMCC 1.2666T= JCM 34827T) as the type strains, respectively.
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