| 著者 |
Jiao D, Chen H, Dai J, Wang J, Wang X, Zhang Y, Liu Y, Wang S, Li W, Jiang A, Zeng H, Qiu D.
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| Abstract |
An ovoid-shaped, Gram-negative, aerobic bacterium, designated M-36T, was isolated from the activated sludge of an industrial wastewater treatment plant in Hefei City, China. Phylogenetic analysis based on comparative 16S rRNA gene sequence alignments showed that strain M-36T is most closely related to bacteria of the family Sphingosinicellaceae and most closely related to species of the genus Pedomonas (95.7%, type species Pedomonas mirosovicensis), while the 16S rRNA gene sequence similarity values to the type strains of species of other genera of Sphingosinicellaceae were observed to be lower than 94.0%. The cells of strain M-36T were observed to be 0.8 µm long and 0.3 µm in diameter. The organism was found to grow at 16-40 °C (optimum, 29 °C), pH 7 and with 0% NaCl (w/v). The major cellular fatty acids were determined to be summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c), summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and C16 : 0. The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two kinds of aminolipids. Chemotaxonomic analysis of strain M-36T indicated that the sole respiratory quinone was ubiquinone-10. The genome sequence of strain M-36T was determined to be 3,691,008 bp with a G+C content of 67.1 mol%. The average nucleotide identity, digital DNA-DNA hybridization values, average amino acid identity and the percentage of conserved proteins between strain M-36T and other genera within the family Sphingosinicellaceae were 70.3-75.0%, 18.0-20.3%, 60.3-69.6% and 42.3-54.3%, respectively. The phylogenetic, genotypic, phenotypic and chemotaxonomic data demonstrate that strain M-36T represents a novel species in a novel genus within the family Sphingosinicellaceae, for which the name Parapedomonas caeni gen. nov., sp. nov. is proposed. Strain M-36T (=CCTCC AB 2024093T=KCTC 8598 T) is the type and only strain of P. caeni.
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