Reference - Detail
| RRC ID | 89663 |
|---|---|
| Author | Williams W, Phan K, Chen J, Legewie S, Kamenz J, Hauf S. |
| Title | Chromosome segregation synchrony in S. pombe is noise limited and arises without positive feedback. |
| Journal | J Cell Biol |
| Abstract |
Anaphase is a key cell cycle transition that ensures faithful genome inheritance. At anaphase onset, sister chromatids separate abruptly and synchronously upon activation of the protease separase. Major cell cycle transitions often involve positive feedback, which contributes to their abruptness and irreversibility; however, whether such feedback is required for anaphase remains unclear. Here, we analyze sister chromatid separation dynamics in fission yeast using high-resolution live-cell imaging and computational modeling. We find that anaphase synchrony relies on fast degradation of the separase inhibitor securin but does not require separase-mediated positive feedback. Hence, sister chromatid separation, being inherently irreversible, may be one of the few major cell cycle transitions that can proceed without positive feedback. A stochastic model fitted to the data revealed that separation synchrony is limited by stochasticity resulting from small-number effects. Together, these results support a feedback-independent mechanism for anaphase onset and identify molecular noise as a fundamental constraint on its temporal precision. |
| Volume | 225(7) |
| Published | 2026-7-6 |
| DOI | 10.1083/jcb.202602088 |
| PII | 282557 |
| PMID | 42132940 |
| PMC | PMC13175033 |
| MeSH | Anaphase Chromatids / genetics Chromatids / metabolism Chromosome Segregation* Feedback, Physiological* Schizosaccharomyces* / cytology Schizosaccharomyces* / genetics Schizosaccharomyces* / metabolism Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism Securin / genetics Securin / metabolism Separase / genetics Separase / metabolism Stochastic Processes |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Bluesky |
| Total number of mentions | 22 |
| Altmetric score changes over past 6months | 6.1 |
| Resource | |
| Yeast | FY15657 |