| RRC ID |
89681
|
| Author |
Stracke R, Jahns O, Keck M, Tohge T, Niehaus K, Fernie AR, Weisshaar B.
|
| Title |
Analysis of PRODUCTION OF FLAVONOL GLYCOSIDES-dependent flavonol glycoside accumulation in Arabidopsis thaliana plants reveals MYB11-, MYB12- and MYB111-independent flavonol glycoside accumulation.
|
| Journal |
New Phytol
|
| Abstract |
The flavonol branch of flavonoid biosynthesis is under transcriptional control of the R2R3-MYBs production of flavonol glycoside1 (PFG1/MYB12, PFG2/MYB11 and PFG3/MYB111) in Arabidopsis thaliana. Here, we investigated the influence of specific PFG transcription factors on flavonol distribution in various organs. A combination of genetic and metabolite analysis was used to identify transcription factor gene-metabolite correlations of the flavonol metabolic pathway. Flavonol glycoside accumulation patterns have been analysed in wild-type and multiple R2R3-MYB PFG mutants in an organ- and development-dependent manner using high-performance thin-layer chromatography, supplemented with liquid chromatography-mass spectroscopy metabolite profiling. Our results clearly demonstrate a differential influence of MYB11, MYB12 and MYB111 on the spatial accumulation of specific flavonol derivatives in leaves, stems, inflorescences, siliques and roots. In addition, MYB11-, MYB12- and MYB111-independent flavonol glycoside accumulation was observed in pollen grains and siliques/seeds. The highly complex tissue- and developmental-specific regulation of flavonol biosynthesis in A. thaliana is orchestrated by at least four PFG transcription factors, differentially influencing the spatial accumulation of specific flavonol derivatives. We present evidence that a separate flavonol control mechanism might be at play in pollen.
|
| Volume |
188(4)
|
| Pages |
985-1000
|
| Published |
2010-12-1
|
| DOI |
10.1111/j.1469-8137.2010.03421.x
|
| PII |
NPH3421
|
| PMID |
20731781
|
| MeSH |
Arabidopsis / cytology
Arabidopsis / enzymology
Arabidopsis / genetics
Arabidopsis / metabolism*
Arabidopsis Proteins / metabolism*
Chromatography, Thin Layer
Flavonols / metabolism*
Genes, Plant / genetics
Glucuronosyltransferase / metabolism
Glycosides / metabolism*
Mutation / genetics
Organ Specificity
Pollen / cytology
Pollen / metabolism
Staining and Labeling
Transcription Factors / metabolism
Transfection
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
pst16145 |
