Reference - Detail
| RRC ID | 89681 |
|---|---|
| Author | Stracke R, Jahns O, Keck M, Tohge T, Niehaus K, Fernie AR, Weisshaar B. |
| Title | Analysis of PRODUCTION OF FLAVONOL GLYCOSIDES-dependent flavonol glycoside accumulation in Arabidopsis thaliana plants reveals MYB11-, MYB12- and MYB111-independent flavonol glycoside accumulation. |
| Journal | New Phytol |
| Abstract |
The flavonol branch of flavonoid biosynthesis is under transcriptional control of the R2R3-MYBs production of flavonol glycoside1 (PFG1/MYB12, PFG2/MYB11 and PFG3/MYB111) in Arabidopsis thaliana. Here, we investigated the influence of specific PFG transcription factors on flavonol distribution in various organs. A combination of genetic and metabolite analysis was used to identify transcription factor gene-metabolite correlations of the flavonol metabolic pathway. Flavonol glycoside accumulation patterns have been analysed in wild-type and multiple R2R3-MYB PFG mutants in an organ- and development-dependent manner using high-performance thin-layer chromatography, supplemented with liquid chromatography-mass spectroscopy metabolite profiling. Our results clearly demonstrate a differential influence of MYB11, MYB12 and MYB111 on the spatial accumulation of specific flavonol derivatives in leaves, stems, inflorescences, siliques and roots. In addition, MYB11-, MYB12- and MYB111-independent flavonol glycoside accumulation was observed in pollen grains and siliques/seeds. The highly complex tissue- and developmental-specific regulation of flavonol biosynthesis in A. thaliana is orchestrated by at least four PFG transcription factors, differentially influencing the spatial accumulation of specific flavonol derivatives. We present evidence that a separate flavonol control mechanism might be at play in pollen. |
| Volume | 188(4) |
| Pages | 985-1000 |
| Published | 2010-12-1 |
| DOI | 10.1111/j.1469-8137.2010.03421.x |
| PII | NPH3421 |
| PMID | 20731781 |
| MeSH | Arabidopsis / cytology Arabidopsis / enzymology Arabidopsis / genetics Arabidopsis / metabolism* Arabidopsis Proteins / metabolism* Chromatography, Thin Layer Flavonols / metabolism* Genes, Plant / genetics Glucuronosyltransferase / metabolism Glycosides / metabolism* Mutation / genetics Organ Specificity Pollen / cytology Pollen / metabolism Staining and Labeling Transcription Factors / metabolism Transfection |
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| Total number of mentions | 0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | pst16145 |