論文 - 詳細
| RRC ID | 89800 |
|---|---|
| 著者 | Kajiura H, Yoshizawa T, Tokumoto Y, Suzuki N, Takeno S, Takeno KJ, Yamashita T, Tanaka SI, Kaneko Y, Fujiyama K, Matsumura H, Nakazawa Y. |
| タイトル | Structure-function studies of ultrahigh molecular weight isoprenes provide key insights into their biosynthesis. |
| ジャーナル | Commun Biol |
| Abstract |
Some plant trans-1,4-prenyltransferases (TPTs) produce ultrahigh molecular weight trans-1,4-polyisoprene (TPI) with a molecular weight of over 1.0 million. Although plant-derived TPI has been utilized in various industries, its biosynthesis and physiological function(s) are unclear. Here, we identified three novel Eucommia ulmoides TPT isoforms-EuTPT1, 3, and 5, which synthesized TPI in vitro without other components. Crystal structure analysis of EuTPT3 revealed a dimeric architecture with a central hydrophobic tunnel. Mutation of Cys94 and Ala95 on the central hydrophobic tunnel no longer synthesizd TPI, indicating that Cys94 and Ala95 were essential for forming the dimeric architecture of ultralong-chain TPTs and TPI biosynthesis. A spatiotemporal analysis of the physiological function of TPI in E. ulmoides suggested that it is involved in seed development and maturation. Thus, our analysis provides functional and mechanistic insights into TPI biosynthesis and uncovers biological roles of TPI in plants. |
| 巻・号 | 4(1) |
| ページ | 215 |
| 公開日 | 2021-2-16 |
| DOI | 10.1038/s42003-021-01739-5 |
| PII | 10.1038/s42003-021-01739-5 |
| PMID | 33594248 |
| PMC | PMC7887238 |
| MeSH | Dimethylallyltranstransferase / chemistry Dimethylallyltranstransferase / genetics Dimethylallyltranstransferase / metabolism* Eucommiaceae / enzymology* Eucommiaceae / genetics Hemiterpenes / biosynthesis* Hemiterpenes / chemistry Latex / biosynthesis* Latex / chemistry Models, Molecular Molecular Weight Mutation Plant Proteins / genetics Plant Proteins / metabolism* Plants, Genetically Modified / enzymology* Plants, Genetically Modified / genetics Protein Conformation Structure-Activity Relationship |
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オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | rpc00001 |