Reference - Detail
| RRC ID | 963 |
|---|---|
| Author | Yoshida S, Katayama E, Kuwae A, Mimuro H, Suzuki T, Sasakawa C. |
| Title | Shigella deliver an effector protein to trigger host microtubule destabilization, which promotes Rac1 activity and efficient bacterial internalization. |
| Journal | EMBO J |
| Abstract |
Shigella deliver a subset of effectors into the host cell via the type III secretion system, that stimulate host cell signal pathways to modulate the actin dynamics required for invasion of epithelial cells. Here we show that one of the Shigella effectors, called VirA, can interact with tubulin to promote microtubule (MT) destabilization, and elicit protrusions of membrane ruffling. Under in vitro conditions, VirA inhibited polymerization of tubulin and stimulated MT destabilization. Upon microinjection of VirA into HeLa cells, a localized membrane ruffling was induced rapidly. Overexpression of VirA in host cells caused MT destruction and protruding membrane ruffles which were absent when VirA was co-expressed with a dominant-negative Rac1 mutant. Indeed, Shigella but not the virA mutant stimulated Rac1, including the formation of membrane ruffles in infected cells. Importantly, the MT structure beneath the protruding ruffling was destroyed. Furthermore, drug-induced MT growth in HeLa cells greatly enhanced the Shigella entry. These results indicate that VirA is a novel type of bacterial effector capable of inducing membrane ruffling through the stimulation of MT destabilization. |
| Volume | 21(12) |
| Pages | 2923-35 |
| Published | 2002-6-17 |
| DOI | 10.1093/emboj/cdf319 |
| PMID | 12065406 |
| PMC | PMC126072 |
| MeSH | Actins / metabolism Animals Bacterial Proteins / genetics Bacterial Proteins / physiology* Brain Chemistry COS Cells Cattle Cell Surface Extensions / metabolism Dimerization HeLa Cells Humans Microscopy, Fluorescence Microtubules / metabolism* Models, Biological Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Shigella / genetics Shigella / metabolism* Shigella / pathogenicity Tubulin / metabolism* Virulence Virulence Factors* rac1 GTP-Binding Protein / genetics rac1 GTP-Binding Protein / metabolism* |
| IF | 9.889 |
| Times Cited | 93 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 27 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Pathogenic microorganisms | S. deliver? |