論文 - 詳細
| RRC ID | 976 |
|---|---|
| 著者 | Kodama T, Akeda Y, Kono G, Takahashi A, Imura K, Iida T, Honda T. |
| タイトル | The EspB protein of enterohaemorrhagic Escherichia coli interacts directly with alpha-catenin. |
| ジャーナル | Cell Microbiol |
| Abstract |
Enterohaemorrhagic Escherichia coli (EHEC) belongs to a family of pathogens that cause attaching and effacing (A/E) lesion on target cells. The EspB protein of EHEC is translocated both to the host cell cytoplasm and to the membrane, and is essential for the signalling events leading to A/E lesion. To determine the actual role of EspB in this process, we tried to identify the EspB binding partner of the host cell protein, using a yeast two-hybrid assay, and obtained a cytoskeletal-associated protein, alpha-catenin. The alpha-catenin bound directly to the N-terminal region of EspB, both in solid (overlay assay) and solution (pull-down assay) phases, and it was recruited to the EHEC adherence site, dependent on EspB. Expression of the N-terminal region of EspB, as well as the whole EspB in host cells, inhibited F-actin accumulation on the adherence site. We conclude that EspB recruits alpha-catenin at the EHEC adherence site by direct interaction, and that the recruitment of alpha-catenin is essential for EHEC-induced A/E lesion formation. |
| 巻・号 | 4(4) |
| ページ | 213-22 |
| 公開日 | 2002-4-1 |
| DOI | 10.1046/j.1462-5822.2002.00176.x |
| PMID | 11952638 |
| MeSH | Bacterial Outer Membrane Proteins / metabolism* Cells, Cultured Cytoskeletal Proteins / chemistry Cytoskeletal Proteins / genetics Cytoskeletal Proteins / metabolism* Escherichia coli O157 / metabolism* Escherichia coli O157 / pathogenicity Escherichia coli Proteins / metabolism* HeLa Cells Humans Recombinant Proteins / metabolism alpha Catenin beta-Galactosidase / analysis |
| IF | 3.43 |
| 引用数 | 68 |
| WOS 分野 | MICROBIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 病原微生物 | RIMD 0509952 |