| RRC ID |
979
|
| 著者 |
Shimizu Y, Yamamichi N, Saitoh K, Watanabe A, Ito T, Yamamichi-Nishina M, Mizutani M, Yahagi N, Suzuki T, Sasakawa C, Yasugi S, Ichinose M, Iba H.
|
| タイトル |
Kinetics of v-src-induced epithelial-mesenchymal transition in developing glandular stomach.
|
| ジャーナル |
Oncogene
|
| Abstract |
The oncogene function in primary epithelial cells is largely unclear. Recombination organ cultures in combination with the stable and transient gene transfer techniques by retrovirus and electroporation, respectively, enable us to transfer oncogenes specifically into primary epithelial cells of the developing avian glandular stomach (proventriculus). In this system, the epithelium and mesenchyme are mutually dependent on each other for their growth and differentiation. We report here that either stable or transient expression of v-src in the epithelium causes budding and migration of epithelial cells into mesenchyme. In response to the transient expression of v-Src or a constitutive active mutant of MEK, we observed immediate downregulation of the Sonic hedgehog gene and subsequent elimination of E-cadherine expression in migrating cells, suggesting the involvement of MAP kinase signaling pathway in these processes. v-src-expressing cells that were retained in the epithelium underwent apoptosis (anoikis) and detached from the culture. Continuous expression of v-src by, for example, Rous sarcoma virus (RSV) was required for the epithelial cells to acquire the ability to express type I collagen and fibronectin genes (mesenchymal markers), and finally to establish the epithelial-mesenchymal transition. These observations would partly explain why RSV does not apparently cause carcinoma formation, but induces sarcomas exclusively.
|
| 巻・号 |
22(6)
|
| ページ |
884-93
|
| 公開日 |
2003-2-13
|
| DOI |
10.1038/sj.onc.1206174
|
| PII |
1206174
|
| PMID |
12584568
|
| MeSH |
Animals
Avian Sarcoma Viruses / metabolism
Cell Transformation, Neoplastic / metabolism
Chick Embryo
Coturnix
Epithelium / metabolism*
Gastric Mucosa / metabolism*
Gene Expression Profiling
Gene Transfer Techniques
Kinetics
Mesoderm / metabolism*
Oncogene Protein pp60(v-src) / metabolism*
Protein Serine-Threonine Kinases / metabolism
Signal Transduction / physiology
Stomach / cytology
|
| IF |
7.971
|
| 引用数 |
16
|
|
WOS 分野
|
GENETICS & HEREDITY
ONCOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 病原微生物 |
|
