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  • 検索条件 : 絞込み (MeSH = Mutagenesis, Insertional / methods*)
生物種 リソース名 タイトル
メダカ Transposon-mediated enhancer trapping in medaka.
酵母 BYP5041 , BYP5042 New plasmids for the disruption and repeated use of selection markers in Saccharomyces cerevisiae.
原核生物(枯草菌) MBS959 , MBS960 , MBS961 , MBS962 , MBS963 , MBS964 , MBS965 , MBS966 , MBS967 , MBS968 , ... Genetic transfer of large DNA inserts to designated loci of the Bacillus subtilis 168 genome.
ヒト・動物細胞 CHO-K1 Targeted knock-in of an scFv-Fc antibody gene into the hprt locus of Chinese hamster ovary cells using CRISPR/Cas9 and CRIS-PITCh systems.
原核生物(大腸菌) Transposon insertion disruptant A novel, simple, high-throughput method for isolation of genome-wide transposon insertion mutants of Escherichia coli K-12.
原核生物(大腸菌) JW2669-KC Silent mischief: bacteriophage Mu insertions contaminate products of Escherichia coli random mutagenesis performed using suicidal transposon delivery plasmids mobilized by broad-host-range RP4 conjugative machinery.
一般微生物 JCM 1170 A convenient method for multiple insertions of desired genes into target loci on the Escherichia coli chromosome.
原核生物(大腸菌) pMR-7wt Construction and expression of a modular gene encoding bacteriophage T7 RNA polymerase.
原核生物(大腸菌) pAB1001 , pAB1002 , pAB2001 , pAB2002 , pMS246 , pMS252 , pMS255 , pMS266 , pMS272 New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions.
原核生物(大腸菌) pJP5603 , pJP5608 An improved suicide vector for construction of chromosomal insertion mutations in bacteria.
ショウジョウバエ DGRC#109052 Trap a gene and find out its function: toward functional genomics in Drosophila.
ミヤコグサ・ダイズ LORE1 Establishment of a Lotus japonicus gene tagging population using the exon-targeting endogenous retrotransposon LORE1.
細胞性粘菌 G90116 Highly effective removal of floxed Blasticidin S resistance cassettes from Dictyostelium discoideum mutants by extrachromosomal expression of Cre.
細胞性粘菌 S00155 Tagging developmental genes in Dictyostelium by restriction enzyme-mediated integration of plasmid DNA.
遺伝子材料 pUPAT-egfp3ic (RDB07884) , pUPAT-SRV (RDB07885) Suppression of nonsense-mediated mRNA decay permits unbiased gene trapping in mouse embryonic stem cells.
遺伝子材料 pxCANCre (RDB1675) An accumulative site-specific gene integration system using Cre recombinase-mediated cassette exchange.
細胞性粘菌 A novel gene trap method using terminator-REMI and 3' rapid amplification of cDNA ends (RACE) in Dictyostelium.
一般微生物 JCM 3201 New vector system for random, single-step integration of multiple copies of DNA into the Rhodococcus genome.
ゼブラフィッシュ Analysis of genes and genome by the tol2-mediated gene and enhancer trap methods.
原核生物(大腸菌) CV-25(DH5alpha) , pINT-ts , pAH69 , pAH121 , pAH130 Replicon-free and markerless methods for genomic insertion of DNAs in phage attachment sites and controlled expression of chromosomal genes in Escherichia coli.