| RRC ID |
1027
|
| 著者 |
Tanaka J, Suzuki T, Mimuro H, Sasakawa C.
|
| タイトル |
Structural definition on the surface of Helicobacter pylori type IV secretion apparatus.
|
| ジャーナル |
Cell Microbiol
|
| Abstract |
Genetic and functional studies have indicated that the type IV secretion system (TFSS) of Helicobacter pylori forms a secretion complex in the cell envelope that protrudes towards the outside in order to inject CagA protein into gastric epithelial cells. However, the proposed structural model is based on partial amino acid homology with the components of the Agrobacterium tumefaciens TFSS. Therefore, we undertook the identification of the structural features of the TFSS exposed on the surface of H. pylori and found that filamentous structures present on the bacterial surface are related to the secretion apparatus. Using immunofluorescence microscopy with antibodies directed to tyrosine-phosphorylated CagA (pY-CagA) and Hp0532 (VirB7) in the infection assay, pY-CagA signals were detected just below the host cell-attached bacteria, where Hp0532 (VirB7) signals were detected as co-localized, suggesting that the CagA injected into the host cell through the TFSS apparatus is still mostly confined to the areas just below the attached bacteria after being phosphorylated. Furthermore, the filamentous structures on bacterium were found to be associated with Hp0532 (VirB7) or Hp0528 (VirB9), the major components of TFSS, by immunogold electron microscopy. These results strongly suggest that the H. pylori TFSS apparatus is a filamentous macromolecular structure protruding from the bacterial envelope.
|
| 巻・号 |
5(6)
|
| ページ |
395-404
|
| 公開日 |
2003-6-1
|
| DOI |
10.1046/j.1462-5822.2003.00286.x
|
| PII |
286
|
| PMID |
12780777
|
| MeSH |
Antigens, Bacterial / analysis
Bacterial Outer Membrane Proteins / analysis*
Bacterial Outer Membrane Proteins / chemistry
Bacterial Outer Membrane Proteins / metabolism
Bacterial Proteins / analysis
Cells, Cultured
Helicobacter Infections / microbiology
Helicobacter pylori / chemistry*
Helicobacter pylori / cytology
Helicobacter pylori / ultrastructure
Humans
Microscopy, Immunoelectron / methods
Models, Genetic
Mutation
Phosphorylation
Protein Transport
Tyrosine / metabolism
|
| IF |
3.43
|
| 引用数 |
101
|
|
WOS 分野
|
MICROBIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 病原微生物 |
H. pylori 26695 (IID 6171)
H. pylori NCTC 11637(IID 3023) |
