RRC ID 1032
Author Li Y, Kawamura Y, Fujiwara N, Naka T, Liu H, Huang X, Kobayashi K, Ezaki T.
Title Chryseobacterium miricola sp. nov., a novel species isolated from condensation water of space station Mir.
Journal Syst. Appl. Microbiol.
Abstract Classification of strain W3-B1, which was isolated from condensation water in the Russian space laboratory Mir, was investigated by a polyphasic taxonomic approach. Cells of strain W3-B1 were nonmotile, asporogenous, gram-negative slender rods with rounded ends. 16S rRNA gene sequence analysis indicated that organism should be placed in the genus Chryseobacterium. This organism contains menaquinone MK-6 as the predominent isoprenoid quinone and 3-OH iso 17:0 (40%), iso 15:0 (33%) as the major fatty acids. Phylogenetically, the nearest relative of strain W3-B1 is Chryseobacterium meningosepticum with sequence similarity of 98.4%, but DNA-DNA hybridization resulted in similarity values of only 52.3%. The G+C mol% is 34.6 mol%. Based upon results obtained by morphological, biochemical, chemotaxonomic, and molecular methods, strain W3-B1 was clearly distinguishable from other Chryseobacterium species. For these reasons, a novel species of family Flavobacteriaceae is proposed; strain W3-B1(T) (= GTC 862(T) = JCM 11413(T) = DSM 14571(T)) is the type strain.
Volume 26(4)
Pages 523-8
Published 2003-11
DOI 10.1078/072320203770865828
PII S0723-2020(04)70218-0
PMID 14666980
MeSH Base Composition Chryseobacterium / classification* Chryseobacterium / cytology Chryseobacterium / isolation & purification* Chryseobacterium / physiology DNA, Bacterial / chemistry DNA, Bacterial / isolation & purification DNA, Ribosomal / chemistry DNA, Ribosomal / isolation & purification Fatty Acids / analysis Fatty Acids / chemistry Molecular Sequence Data Movement Nucleic Acid Hybridization Phenotype Phylogeny Pigments, Biological / analysis RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Spacecraft* Spores, Bacterial Vitamin K 2 / analysis Vitamin K 2 / chemistry Water Microbiology*
IF 3.899
Times Cited 40
WOS Category BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
Resource
Pathogenic microorganisms JCM 11413?