RRC ID 10740
著者 Tian C, Sen D, Shi H, Foehr ML, Plavskin Y, Vatamaniuk OK, Liu J.
タイトル The RGM protein DRAG-1 positively regulates a BMP-like signaling pathway in Caenorhabditis elegans.
ジャーナル Development
Abstract The bone morphogenetic protein (BMP) signaling pathway regulates multiple developmental and homeostatic processes. Mutations in the pathway can cause a variety of somatic and hereditary disorders in humans. Multiple levels of regulation, including extracellular regulation, ensure proper spatiotemporal control of BMP signaling in the right cellular context. We have identified a modulator of the BMP-like Sma/Mab pathway in C. elegans called DRAG-1. DRAG-1 is the sole member of the repulsive guidance molecule (RGM) family of proteins in C. elegans, and is crucial in regulating body size and mesoderm development. Using a combination of molecular genetic and biochemical analyses, we demonstrate that DRAG-1 is a membrane-associated protein that functions at the ligand-receptor level to modulate the Sma/Mab pathway in a cell-type-specific manner. We further show that DRAG-1 positively modulates this BMP-like pathway by using a novel Sma/Mab-responsive reporter. Our work provides a direct link between RGM proteins and BMP signaling in vivo and a simple and genetically tractable system for mechanistic studies of RGM protein regulation of BMP pathways.
巻・号 137(14)
ページ 2375-84
公開日 2010-7-1
DOI 10.1242/dev.051615
PII dev.051615
PMID 20534671
PMC PMC2889605
MeSH Animals Bone Morphogenetic Proteins / genetics Bone Morphogenetic Proteins / metabolism Bone Morphogenetic Proteins / physiology* Caenorhabditis elegans* / genetics Caenorhabditis elegans* / growth & development Caenorhabditis elegans* / metabolism Humans Membrane Proteins / genetics Membrane Proteins / metabolism* Mutation Neurons / metabolism Protein Binding / genetics Proteins / genetics Proteins / metabolism Signal Transduction / genetics*
IF 5.611
引用数 21
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
線虫 tm3773