| RRC ID |
10740
|
| Author |
Tian C, Sen D, Shi H, Foehr ML, Plavskin Y, Vatamaniuk OK, Liu J.
|
| Title |
The RGM protein DRAG-1 positively regulates a BMP-like signaling pathway in Caenorhabditis elegans.
|
| Journal |
Development
|
| Abstract |
The bone morphogenetic protein (BMP) signaling pathway regulates multiple developmental and homeostatic processes. Mutations in the pathway can cause a variety of somatic and hereditary disorders in humans. Multiple levels of regulation, including extracellular regulation, ensure proper spatiotemporal control of BMP signaling in the right cellular context. We have identified a modulator of the BMP-like Sma/Mab pathway in C. elegans called DRAG-1. DRAG-1 is the sole member of the repulsive guidance molecule (RGM) family of proteins in C. elegans, and is crucial in regulating body size and mesoderm development. Using a combination of molecular genetic and biochemical analyses, we demonstrate that DRAG-1 is a membrane-associated protein that functions at the ligand-receptor level to modulate the Sma/Mab pathway in a cell-type-specific manner. We further show that DRAG-1 positively modulates this BMP-like pathway by using a novel Sma/Mab-responsive reporter. Our work provides a direct link between RGM proteins and BMP signaling in vivo and a simple and genetically tractable system for mechanistic studies of RGM protein regulation of BMP pathways.
|
| Volume |
137(14)
|
| Pages |
2375-84
|
| Published |
2010-7-1
|
| DOI |
10.1242/dev.051615
|
| PII |
dev.051615
|
| PMID |
20534671
|
| PMC |
PMC2889605
|
| MeSH |
Animals
Bone Morphogenetic Proteins / genetics
Bone Morphogenetic Proteins / metabolism
Bone Morphogenetic Proteins / physiology*
Caenorhabditis elegans* / genetics
Caenorhabditis elegans* / growth & development
Caenorhabditis elegans* / metabolism
Humans
Membrane Proteins / genetics
Membrane Proteins / metabolism*
Mutation
Neurons / metabolism
Protein Binding / genetics
Proteins / genetics
Proteins / metabolism
Signal Transduction / genetics*
|
| IF |
5.611
|
| Times Cited |
21
|
|
WOS Category
|
DEVELOPMENTAL BIOLOGY
|
| Resource |
| C.elegans |
tm3773 |
